Vaginal swabs versus lavage for detection of Trichomonas vaginalis and bacterial vaginosis among HIV-positive women
Vaginal swabs versus lavage for detection of Trichomonas vaginalis and bacterial vaginosis among HIV-positive women
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DOI：
10.1097/01.olq.0000151416.56717.7d
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Martin, DH
中科院分区：
文献类型：
--
作者：
Kissinger, PJ;Dumestre, J;Martin, DH
目的：宫颈阴道灌洗 (CVL) 常用于研究，作为标本采集方法，可能比阴道拭子更容易、更准确。 目标：本研究的目的是比较 (CVL) 与阴道拭子对细菌性阴道病 (BV) 和阴道毛滴虫 (TV) 的检测。研究：从 216 名 HIV 感染者中采集了 CVL 和阴道拭子妇女。通过 CVL 和拭子采集方法，使用 TV 湿涂片和 Amsel BV 标准进行临床评估。使用的实验室金标准是 Nugent 的 BV 标准和通过拭子收集的 TV 培养物的 InPouch（Biomed Diagnostics，圣何塞，加利福尼亚州）。结果：根据金标准，BV 的患病率为 49.3%，TV 的患病率为 25.2%。直接显微镜检查与 TV 培养的灵敏度相比，CVL 为 72.2，阴道拭子为 52.8（P < 0.05）。 Amsel 与 Nugent 的 BV 标准的敏感性分别为：CVL 为 36.2，阴道拭子为 34.0（P < 0.80）。 CVL 与阴道拭子对于 BV 和 TV 的 Kappa 得分均非常一致。结论：对于这 2 种下生殖道感染，CVL 作为标本采集方法与阴道拭子相当，并且可能更适合诊断 TV。
Objectives: Cervicovaginal lavage (CVL) is often used for research and may be easier and more accurate than vaginal swabs as a specimen collection method.Goal: The goal of this study was to compare (CVL) with vaginal swabs for the detection of bacterial vaginosis (BV) and Trichomonas vaginalis (TV).Study: CVL and vaginal swabs were collected from 216 HIV-infected women. Clinical assessments were made using wet mount for TV and Amsel's criteria for BV through CVL and swab collection methods. Laboratory gold standards used were Nugent's criteria for BV and InPouch (Biomed Diagnostics, San Jose, CA) culture for TV collected by swab.Results: The prevalence by gold standards for BV was 49.3% and for TV was 25.2%. Sensitivities for direct microscopy versus culture for TV were 72.2 for CVL and 52.8 for vaginal swab (P < 0.05). Sensitivities for Amsel's versus Nugent's criteria for BV were 36.2 for CVL and 34.0 for vaginal swab (P < 0.80). Kappa scores of agreement between CVL and vaginal swabs for BV and TV were excellent for both.Conclusion: CVL was comparable to vaginal swabs as a specimen collection method for these 2 lower genital tract infections and may be superior for the diagnosis of TV.