MiR-320 and miR-494 affect cell cycles of primary murine bronchial epithelial cells exposed to benzo[a]pyrene
MiR-320 and miR-494 affect cell cycles of primary murine bronchial epithelial cells exposed to benzo[a]pyrene
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DOI：
10.1016/j.tiv.2009.11.013
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发表时间：
2010-04-01
影响因子：
3.2
通讯作者：
Wu, Yan
中科院分区：
文献类型：
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作者：
Duan, Huihan;Jiang, Yiguo;Wu, Yan
MicroRNAs （miRNAs）是一类对各种生物过程具有深远影响的非编码小RNA分子。一些mirna通过调控细胞周期进程参与肿瘤发生。在这里，我们培养了原代小鼠支气管上皮细胞，然后检测了暴露于苯并[a]芘（B[a]P）的细胞中miR-320和miR-494的表达。为了更好地表征miR-320和miR-494在细胞周期进程中的作用，我们使用miRNA抑制剂下调miRNA的表达，并通过流式细胞术分析确定细胞周期分布和细胞周期蛋白依赖性激酶6 （CDK6）的表达。用1 μ M - Mall处理细胞24小时导致miR-320和miR-494表达的时间依赖性增加。此外，在处理的细胞中显示G1阻滞和CDK6表达下调。流式细胞术分析显示，在暴露于B细胞中抑制miR-320和miR-494的表达后，Cl骤停得到缓解，CDK6的表达升高[a]。这些结果表明，调控B[a]P暴露细胞周期进程的miRNA-320和miR-494的表达水平可能通过CDK6影响G1/S转变，并进一步揭示mirna在暴露于B[a]P的小鼠支气管上皮细胞的细胞周期中的功能。2009爱思唯尔有限公司版权所有。
MicroRNAs (miRNAs) are a class of small noncoding RNA molecules with profound impact on various biological processes. Some miRNAs are involved in tumorigenesis by regulation of cell cycle progression. Here, we cultured primary murine bronchial epithelial cells and then examined the expression of miR-320 and miR-494 in cells exposed to benzo[a]pyrene (B[a]P). To better characterize roles of miR-320 and miR-494 in cell cycle progression, we used miRNA inhibitors to downregulate expression of miRNAs and determined cell cycle distribution and expression of cyclin-dependent kinases 6 (CDK6) by flow cytometric analysis. Treating cells with 1 mu M Mall) for 24 h resulted in time-dependent increases in miR-320 and miR-494 expression. Moreover, G1 arrest and downregulated expression of CDK6 were shown in the treated cells. Flow cytometric analysis indicated a relief of Cl arrest and an elevated expression of CDK6 after inhibition of the expressions of miR-320 and miR-494 in cells exposed to B[a]P. These results suggest that expression levels of miRNA-320 and miR-494, which regulate B[a]P-exposed cell cycle progression, may impact G1/S transition through CDK6, and provide further insights into functions of miRNAs in cell cycle of primary murine bronchial epithelial cells exposed to B[a]P. (C) 2009 Elsevier Ltd. All rights reserved.