CHROMOSOME SIZE POLYMORPHISMS IN PLASMODIUM-FALCIPARUM CAN INVOLVE DELETIONS AND ARE FREQUENT IN NATURAL PARASITE POPULATIONS
CHROMOSOME SIZE POLYMORPHISMS IN PLASMODIUM-FALCIPARUM CAN INVOLVE DELETIONS AND ARE FREQUENT IN NATURAL PARASITE POPULATIONS
复制标题
DOI：
10.1016/0092-8674(86)90487-3
复制
发表时间：
1986-01-01
期刊：
影响因子：
64.5
通讯作者：
KEMP, DJ
中科院分区：
文献类型：
--
作者：
CORCORAN, LM;FORSYTH, KP;KEMP, DJ
对恶性疟原虫独立培养分离株的比较表明，虽然染色体数目恒定，但类似染色体的大小差异很大。我们在这里表明，在无性血液阶段的分化过程中不会产生染色体大小多态性，因为克隆寄生虫系的分子核型在生命周期的这一部分中是恒定的。使用整个恶性疟原虫染色体作为杂交探针来检查两个独立寄生虫种群内的多态性的实验表明，此处观察到的多态性不是大规模染色体间交换的结果，并且暗示缺失/重复代表了产生染色体长度多态性的一种模式。尽管缺失可能涉及重复的 DNA，但我们在此表明恶性疟原虫抗原的结构基因也可能丢失。此外，这些引人注目的大小多态性不仅发生在恶性疟原虫的培养品系中，而且在自然疟疾感染中也以惊人的频率发生。
A comparison of independent cultured isolates of Plasmodium falciparum revealed that while chromosome number was constant, the sizes of analogous chromosome varied widely. We show here that chromosome size polymorphisms are not generated during differentiation of the asexual blood stages, as the molecular karyotype of a cloned parasite line is constant through this part of the life cycle. Experiments using whole P. falciparum chromosomes as hybridization probes to examine polymorphisms within two independent parasite populations indicate that the polymorphisms observed here are not the consequence of large-scale interchromsomal exchanges, and imploy that deletions/duplications represent one mode of generating chromsome length polymorphisms. Although the deletions probably involve repetitive DNA, we show here that structural genes for P. falciparum antigens can also be lost. Furthermore, these dramatic size polymorphisms occur not only in cultured lines of P. faliciparum, but with suprising frequency in natural malarial infections.