A NOVEL HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROTEIN, TEV, SHARES SEQUENCES WITH TAT, ENV, AND REV PROTEINS
A NOVEL HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROTEIN, TEV, SHARES SEQUENCES WITH TAT, ENV, AND REV PROTEINS
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DOI：
10.1128/jvi.64.6.2505-2518.1990
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发表时间：
1990-06-01
影响因子：
5.4
通讯作者：
FELBER, BK
中科院分区：
文献类型：
--
作者：
BENKO, DM;SCHWARTZ, S;FELBER, BK
我们在人类免疫缺陷病毒1型感染的细胞中检测到一种新的28千道尔顿蛋白p28tev。Tev可被tat和rev单特异性抗体识别。tev起始于第一个AUG，在其氨基端包含tat的第一个外显子，在中间包含env的一小部分，在其羧基端包含rev的第二个外显子。克隆了一个产生tev的cDNA克隆并在人细胞中表达。序列分析表明，tev mRNA是通过剪接到位于env区的新外显子而产生的。这鉴定了第四类多重剪接的人类免疫缺陷病毒mrna，在感染和转染的细胞中产生，tev在病毒生命周期中受到调节，类似于其他调节蛋白，tat， rev和nef，并在功能测定中显示tat和rev活性。由于tev含有tat和rev的重要功能域，并且在转染后很早就产生，因此它可能是病毒表达初始阶段的重要调节因子。另一种含有env和rev序列的rev相关蛋白p186Drev被鉴定为不具有可检测到的rev活性。
We have characterized a novel 28-kilodalton protein, p28tev, detected in human immunodeficiency virus type 1-infected cells. tev is recognized by both tat and rev monospecific antibodies. tev is initiated at the tat AUG and contains the first exon of tat at its amino terminus, a small portion of env in the middle, and the second exon of rev at its carboxy terminus. A cDNA clone producing tev was cloned and expressed in human cells. Sequence analysis revealed that the tev mRNA is generated by splicing to a novel exon located in the env region. This identifies a fourth class of multiply spliced human immunodeficiency virus mRNAs, produced in infected and transfected cells, tev is regulated during the virus life cycle similarly to the other regulatory proteins, tat, rev, and nef, and displays both tat and rev activities in functional assays. Since tev contains important functional domains of tat and rev and is produced very early after transfection, it may be an important regulator in the initial phase of virus expression. Another rev-related protein, p186Drev, containing env and rev sequences, was characterized and was found not to have detectable rev activity.