Intratumoral macrophage phenotype and CD8+ T lymphocytes as potential tools to predict local tumor outgrowth at the intervention site in malignant pleural mesothelioma
Intratumoral macrophage phenotype and CD8+ T lymphocytes as potential tools to predict local tumor outgrowth at the intervention site in malignant pleural mesothelioma
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DOI：
10.1016/j.lungcan.2015.03.013
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发表时间：
2015-06-01
期刊：
影响因子：
5.3
通讯作者：
Aerts, Joachim G. J. V.
中科院分区：
文献类型：
--
作者：
Cornelissen, Robin;Lieyense, Lysanne A.;Aerts, Joachim G. J. V.
目的：在恶性胸膜间皮瘤（MPM）患者中，侵入性手术后的局部肿瘤生长（LTO）是一种众所周知的并发症。目前，没有生物标志物可用于预测LTO的发生。本研究旨在探讨肿瘤巨噬细胞浸润和CD 8（+）T细胞的表型和/或浸润是否预测LTO.Materials和方法：10例发生LTO的间皮瘤患者与10例非LTO间皮瘤患者的临床和病理匹配。对诊断性活检进行免疫组化以确定总TAM（CD 68）、M2 TAM（CD 163）和CD 8（+）T细胞计数（CD 8）。结果：两组之间的平均M2/总TAM比率不同：LTO组为0.90 +/- 0.09，而非LTO患者为0.63 +/- 0.09（p < 0.001）。此外，两组之间的平均CD 8（+）T细胞计数存在显著差异：30/0.025 cm（2）（范围2-60）和140/0.025 cm（2）（范围23-314），无LTO的患者（p < 0.01）。这项研究表明，局部干预后发生LTO的患者具有较高的M2/总TAM比率和较低的CD 8（+）诊断时的细胞计数与未发生这种生长的患者相比。我们认为，M2/总TAM比值和CD 8（+）T细胞数量是预测MPM患者易于发生LTO的潜在工具。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Objectives: In patients with malignant pleural mesothelioma (MPM), local tumor outgrowth (LTO) after invasive procedures is a well-known complication. Currently, no biomarker is available to predict the occurrence of LTO. This study aims to investigate whether the tumor macrophage infiltration and phenotype of and/or the infiltration of CD8(+) T-cells predicts LTO.Materials and methods: Ten mesothelioma patients who developed LTO were clinically and pathologically matched with 10 non-LTO mesothelioma patients. Immunohistochemistry was performed on diagnostic biopsies to determine the total TAM (CD68), the M2 TAM (CD163) and CD8(+) T-cell count (CD8).Results: The mean M2/total TAM ratio differed between the two groups: 0.90 +/- 0.09 in the LTO group versus 0.63 +/- 0.09 in patients without LTO (p < 0.001). In addition, the mean CD8(+) T-cell count was significantly different between the two groups: 30 per 0.025 cm(2) (range 2-60) in the LTO group and 140 per 0.025 cm(2) (range 23-314) in the patients without LTO (p < 0.01).Conclusion: This study shows that patients who develop LTO after a local intervention have a higher M2/total TAM ratio and lower CD8(+) cell count at diagnosis compared to patients who did not develop this outgrowth. We propose that the M2/total TAM ratio and the CD8(+) T-cell amount are potential tools to predict which MPM patients are prone to develop LTO. (C) 2015 Elsevier Ireland Ltd. All rights reserved.