DISEASE RESPONSE TO TOBACCO MOSAIC-VIRUS IN TRANSGENIC TOBACCO PLANTS THAT CONSTITUTIVELY EXPRESS THE PATHOGENESIS-RELATED PR1B GENE
DISEASE RESPONSE TO TOBACCO MOSAIC-VIRUS IN TRANSGENIC TOBACCO PLANTS THAT CONSTITUTIVELY EXPRESS THE PATHOGENESIS-RELATED PR1B GENE
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DOI：
10.1016/0042-6822(89)90224-9
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发表时间：
1989-11-01
期刊：
影响因子：
3.7
通讯作者：
KLESSIG, DF
中科院分区：
文献类型：
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作者：
CUTT, JR;HARPSTER, MH;KLESSIG, DF
在某些烟草品种中，致病相关（PR）基因PR1a、PR1b和PR1c诱导的时空模式与病毒感染的相关性表明，PR蛋白与病毒抗病有关。为了验证烟草PR1蛋白是否与病毒抗性有关，构建了组成型表达PR1b基因的转基因烟草植株。这种蛋白从转基因植物的细胞中分泌出来，并在细胞外空间积累，其水平与非转基因植物中发现的水平相当，与抗病性有关。由该品种衍生的转基因植株（cv.）Xanthi（对烟草花叶病毒[TMV]感染易感）在TMV感染后没有延迟发病或减轻全身症状的严重程度。在转基因植物衍生的cv。抗TMV植株的出现时间、大小和一般形态以及产生的病毒病损数与亲本对照植株感染TMV后相似。这些数据表明，烟草的PR1b蛋白不足以产生TMV抗性，并暗示PR1蛋白可能不是唯一的抗病毒因子。
Correlation of the temporal and spacial pattern of induction of the pathogenesis-related (PR) genes PR1a, PR1b, and PR1c with viral infections in certain tobacco cultivars has implicated PR proteins in viral disease resistance. To test whether the PR1 proteins of tobacco are involved in viral resistance, transgenic Nicotiana tabacum plants were constructed which constitutively express the PR1b gene. This protein was secreted from cells of transgenic plants and accumulated in the extracellular space at levels equivalent to those found in nontransgenic plants in association with disease resistance. Transgenic plants derived from the cultivar (cv.) Xanthi (susceptible to tobacco mosaic virus [TMV] infection) exhibited no delayed onset or reduction in the severity of systemic symptoms after TMV infection. In transgenic plants derived from cv. Xanthi-nc (TMV resistant), the time of appearance, the size and general morphology, and the number of viral lesions produced were similar to the parental control plants after TMV infection. These data indicate that the PR1b protein of tobacco is not sufficient for TMV resistance, and imply that the PR1 proteins may not function as unique antiviral factors.