Highly proliferative fibroblasts forming fibrotic focus govern metastasis of invasive ductal carcinoma of the breast
Highly proliferative fibroblasts forming fibrotic focus govern metastasis of invasive ductal carcinoma of the breast
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DOI：
10.1038/modpathol.3880310
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发表时间：
2001-04-01
期刊：
影响因子：
7.5
通讯作者：
Ochiai, A
中科院分区：
文献类型：
--
作者：
Hasebe, T;Sasaki, S;Ochiai, A
我们已经报道了有纤维化灶的浸润性导管癌(IDCs)比没有纤维化灶的IDCs具有更多的侵袭性特征，FF是由成纤维细胞和不同数量的胶原纤维组成的混合物，这表明高增殖的成纤维细胞形成FF会增加IDCs和FF的恶性潜能，本研究的目的是检查形成和不形成FF的成纤维细胞的增殖活性是否存在差异，这在IDCs的关闭发展中起重要作用。用小鼠抗Ki-67抗原的MIB-1单抗免疫组织化学方法检测成纤维细胞的增殖活性，MIB-1标记指数(LI)是形成成纤维细胞的细胞核呈阳性的百分比，每个成纤维细胞计数300个成纤维细胞，比较形成成纤维细胞的增殖活性与淋巴转移(LNM)或远处器官转移(DOM)的关系。多因素分析显示，高MIB-1LI的成纤维细胞形成FF的相对危险性和DOM的危险性显著增加(P
We have already reported that invasive ductal carcinomas (IDCs) with fibrotic focus (FF) have more aggressive characteristics than those without FF, FF is composed of a mixture of fibroblasts and various amounts of collagen fibers, suggesting that highly proliferative fibroblasts forming FF increase the malignant potential of IDCs with FF, The purpose of this study was to examine whether there is a difference of proliferative activity of fibroblasts forming and not forming FF, which plays an important role in the turnoff progression of IDCs, Two hundred three consecutive cases of IDC of the breast surgically treated at the National Cancer Center Hospital East formed the basis for this study. The proliferative activity of the fibroblasts forming the FF was immunohistochemically evaluated bf using mouse MIB-1 monoclonal antibody against Ki-67 antigen, The MIB-1 labeling index (LI) is the percentage of fibroblasts forming FF that have positively stained nuclei, and 300 fibroblasts were counted in each FF, The significance of the proliferative activity of fibroblasts forming FF with regard to lymph hade metastasis (LNM) or distant-organ metastasis (DOM) was compared with well-known prognostic parameters. Multivariate analysis demonstrated that high MIB-1 LI of fibroblasts forming FF significantly increased the relative risk of LNM and the hazard rate of DOM (P