Identification of possible reactive oxygen species involved in ultraviolet radiation-induced oxidative DNA damage
Identification of possible reactive oxygen species involved in ultraviolet radiation-induced oxidative DNA damage
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DOI：
10.1016/s0891-5849(97)00126-3
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发表时间：
1997-01-01
影响因子：
7.4
通讯作者：
Wei, HC
中科院分区：
文献类型：
--
作者：
Zhang, XS;Rosenstein, BS;Wei, HC
我们以前已经证明，紫外线（UV）光谱（UVA，UVB和UVC）的每个区域诱导纯化的小牛胸腺DNA和HeLa细胞中的8-氧代-7，8-二氢-2 '-脱氧鸟苷（8-oxodGuo）的形成，在一个通量依赖性的方式。在本研究中，我们进一步表征了可能的活性氧（ROS）参与诱导8-oxodGuo的紫外辐射。叠氮化钠是一种单线态氧（O-1（2））清除剂，但它对HO有猝灭作用。还报道，抑制8-oxodGuo生产小牛胸腺DNA暴露于UVA，UVB，或UVC在浓度依赖性的方式与最大淬灭效果超过90%，在10 mM的浓度。过氧化氢酶，在50 U/ml的浓度，减少约50%的UVA和UVB诱导的8-oxodGuo的形成的产量，但对UVC诱导的8-oxodGuo生产几乎没有影响。相反，50 U/ml的超氧化物歧化酶（SOD）也不影响诱导8-oxodGuo的任何部分的UV光谱。羟基自由基（HO .）清除剂甘露醇和二甲基亚砜（DMSO）适度降低UVA和UVB诱导的8-oxodGuo水平，但不降低UVC诱导的8-oxodGuo水平。相反，甘露醇和DMSO促进UVC诱导的8-oxodGuo的形成。这些结果表明，某些类型的ROS参与UV诱导的8-oxodGuo形成，其中O-1（2）在整个UV光谱中起主要作用。除UVC外，其他ROS如过氧化氢（H2 O2）和HO。也可能参与UVA和UVB诱导的氧化性DNA损伤。超氧阴离子似乎不参与紫外线诱导的小牛胸腺DNA中鸟苷的氧化，因为SOD没有显示出任何淬灭作用。(C)1997年爱思唯尔科学公司
We have previously demonstrated that each region of the ultraviolet (UV) spectrum (UVA, UVB, and UVC) induces the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in purified calf thymus DNA and HeLa cells in a fluence-dependent manner. In the present study, we further characterize the possible reactive oxygen species (ROS) that are involved in the induction of 8-oxodGuo by UV radiation. Sodium azide, a singlet oxygen (O-1(2)) scavenger though its quenching effect on HO . was also reported, inhibited 8-oxodGuo production in calf thymus DNA exposed to UVA, UVB, or UVC in a concentration-dependent fashion with maximal quenching effect of over 90% at a concentration of 10 mM. Catalase, at a concentration of 50 U/ml, reduced the yields of UVA-and UVB-induced 8-oxodGuo formation by approximately 50%, but had little effect on UVC-induced 8-oxodGuo production. In contrast, 50 U/ml of superoxide dismutase (SOD) did nor affect induction of 8-oxodGuo by any portion of the UV spectrum. Hydroxyl radical (HO .) scavengers mannitol and dimethylsulfoxide (DMSO) moderately reduced the levels of 8-oxodGuo induced by UVA and UVB, but not those by UVC. instead, mannitol and DMSO enhanced the formation of 8-oxodGuo induced by UVC. These results suggest that certain types of ROS are involved in UV-induced 8-oxodGuo formation with O-1(2) playing the predominant role throughout the UV spectrum. Except for UVC, other ROS such as hydrogen peroxide (H2O2) and HO . may also be involved in UVA- and UVB-induced oxidative DNA damage. Superoxide anion appears not to participate in UV-induced oxidation of guanosine in calf thymus DNA, as SOD did not display any quenching effects. (C) 1997 Elsevier Science Inc.