Nanocrystals modified with peptide nucleic acids (PNAs) for selective self-assembly and DNA detection
Nanocrystals modified with peptide nucleic acids (PNAs) for selective self-assembly and DNA detection
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DOI：
10.1021/ja035399g
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发表时间：
2003-10-15
影响因子：
15
通讯作者：
Klibanov, AM
中科院分区：
文献类型：
--
作者：
Chakrabarti, R;Klibanov, AM
制备了肽核酸修饰的金纳米晶体，并将其应用于自组装和DNA传感。具有不同PNA结构基序的实验表明：（1）PNA合成设计中的多功能性可用于调节纳米晶体的静电表面性质，提供了控制组装速率和聚集体尺寸的机会，（2）短（6个碱基）PNA在附着于纳米颗粒时可有效杂交，提供了产生具有小颗粒间间距的材料的途径，以及（3）PNA的上级碱基对错配选择性在纳米表面上进一步增强，使得PNA修饰的纳米颗粒能够充当高度选择性的纳米级传感器，以及用于无缺陷自组装的电子探针。这最后一个特点是加上DNA杂交后胶体稳定性的实质性变化，以开发一种新的比色DNA测定，检测单碱基缺陷的存在在几分钟内。PNA杂交的各种模式，包括PNA-PNA相互作用的第一个实际应用，被用于指导纳米颗粒组装成宏观排列。与DNA修饰的纳米晶体的类似实验相比，获得了更短的双链体互连和更大的组装特异性。
Gold nanocrystals modified with peptide nucleic acids (PNAs) have been prepared and applied to self-assembly and DNA sensing. Experiments with different PNA structural motifs show that (1) the versatility in PNA synthetic design can be used to modulate the electrostatic surface properties of nanocrystals, presenting an opportunity to control assembly rate and aggregate size, (2) short (6 base) PNAs can hybridize effectively while attached to nanoparticles, providing a route to generating materials with small interparticle spacings, and (3) the superior base pair mismatch selectivity of PNAs is further enhanced on nanosurfaces, enabling PNA-modified nanoparticles to act as highly selective nanoscale sensors, as well as synthons for defect-free self-assembly. This last feature was coupled with a substantial change in colloidal stability upon DNA hybridization to develop a novel colorimetric DNA assay that detects the presence of single base imperfections within minutes. Various modes of PNA hybridization, including the first practical application of PNA-PNA interactions, were used to direct the assembly of nanoparticles into macroscopic arrangements. Shorter duplex interconnects and greater specificity in assembly were obtained compared to similar experiments with DNA-modified nanocrystals.