Daphnane Diterpene Esters with Anti-proliferative Activities against Human Lung Cancer Cells from Daphne genkwa
Daphnane Diterpene Esters with Anti-proliferative Activities against Human Lung Cancer Cells from Daphne genkwa
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DOI：
10.1248/cpb.58.234
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发表时间：
2010-02-01
影响因子：
1.7
通讯作者：
Lee, Sang Kook
中科院分区：
文献类型：
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作者：
Hong, Ji-Young;Nam, Joo-Won;Lee, Sang Kook
从瑞香科瑞香花中分离得到2个新的瑞香烷型二萜酯化合物，分别为元花碱(1)和元花碱(2)，以及3个已知的二萜化合物：元花碱(3)、元花碱(4)和元花根(5)。通过一维和二维核磁共振实验相结合，包括相关光谱(COSY)、异核多量子相关(HMQC)、异核多键相关(HMBC)和旋转框架Overhauser增强光谱(ROESY)序列以及质谱学，确定了它们的结构。所有分离得到的化合物对A549人肺癌细胞和MRC-5人正常肺上皮细胞均有抑制作用。化合物1~5对A549肺癌细胞有较强的抑制增殖作用，IC50值为12~53 nm，而对MRC-5正常肺上皮细胞无细胞毒作用。
Two new daphnane-type diterpene esters, yuanhuahine (1) and yuanhualine (2), were isolated from the flowers of Daphne genkwa (Thymelaeaceae) along with three known diterpene esters, yuanhuacine (3), yuanhuadine (4), and yuanhuagine (5). Their structures were determined by a combination of 1D and 2D NMR experiments, including correlation spectroscopy (COSY), heteronuclear multiple quantum correlation (HMQC), heteronuclear multiple bond correlation (HMBC), and rotating frame Overhauser enhancement spectroscopy (ROESY) sequences, and mass spectrometry. All the isolated compounds were tested against A549 human lung cancer cells and MRC-5 human normal lung epithelial cells. Compounds 1-5 exhibited potent anti-proliferative effects against A549 lung cancer cells with IC50 values of 12-53 nm, whereas these compounds were relatively non-cytotoxic against MRC-5 normal lung epithelial cells.