Differences in the composition of intestinal Bifidobacterium species and the development of allergic diseases in infants in rural Japan
Differences in the composition of intestinal Bifidobacterium species and the development of allergic diseases in infants in rural Japan
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DOI：
10.1111/j.1365-2222.2007.02676.x
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发表时间：
2007-04-01
影响因子：
6.1
通讯作者：
Kohno, Y.
中科院分区：
文献类型：
--
作者：
Suzuki, S.;Shimojo, N.;Kohno, Y.
背景：双歧杆菌是婴儿肠道菌群中的优势菌属，由许多不同的菌种组成。在北方进行的一系列研究表明，过敏婴儿和健康对照组的双歧杆菌属物种组成存在差异。需要进一步的研究，以证实这一observation.Objective：探讨粪便双歧杆菌属过敏的婴儿和健康对照在日本的组成，使用分子methods.Methods：足月出生的婴儿进行了随访6个月的年龄在当地医院在日本农村。根据过敏症状和皮肤点刺试验确定是否存在过敏。使用9种基于16S rDNA序列的双歧杆菌种特异性或组特异性引物，在1、3和6个月龄时评估过敏婴儿（n=10）和健康对照（n=16）的粪便双歧杆菌种。没有发现双歧杆菌的婴儿人数是4 1月龄时为15.4%，3月龄时为7.7%，6月龄时为3.3%，均为健康对照。在1月龄时，小链双歧杆菌组的过敏原检出率明显高于健康对照组（60.0%比6.3%，P < 0.01）。在6个月大时，过敏性婴儿的B患病率较高。两歧组与健康对照组比较差异有显著性（70.0% vs12.5%，P < 0.01）。这些差异不相关的喂养methods.Conclusions：我们的研究结果在日本农村的婴儿支持的假设，肠道双歧杆菌物种的组成差异可能与过敏的发展在婴儿早期，虽然负责的物种可能会有所不同的国家或种族。
Background: Bifidobacterium is a dominant genus in the intestinal microbiota of infants and comprises many different species. A series of studies performed in northern Europe showed differences in the composition of Bifidobacterium species between allergic infants and healthy controls. Additional studies are needed to confirm this observation.Objective: To investigate the composition of fecal Bifidobacterium species in allergic infants and healthy controls in Japan, using molecular methods.Methods: Full-term born babies were followed up to 6 months of age at a local hospital in rural Japan. The presence or absence of allergy was determined based on allergic symptoms and skin prick tests. Fecal Bifidobacterium species in allergic infants (n=10), and healthy controls (n=16) were evaluated using nine Bifidobacterium species-specific or group-specific primers based on 16S rDNA sequences at 1, 3, and 6 months of age.Results: The number of the infants in whom no Bifidobacterium species could be found was four (15.4%) at 1 month, two (7.7%) at 3 months, and one (3.3%) at 6 months of age, all of whom were healthy controls. At 1 month of age, allergic infants had a higher prevalence f the Bifidobacterium catenulatum group than healthy controls (60.0% vs. 6.3%, P < 0.01). At 6 months of age, allergic infants had a higher prevalence of B. bifidum than healthy controls (70.0% vs. 12.5%, P < 0.01). These differences were not related to feeding method.Conclusions: Our results in infants in rural Japan support the hypothesis that a compositional difference in intestinal Bifidobacterium species may be associated with the development of allergy in early infancy, although the responsible species might vary among countries or races.