ID-CUBE direct analysis in real time high-resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.
ID-CUBE direct analysis in real time high-resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.
复制标题
DOI：
10.1002/rcm.6226
复制
发表时间：
2012-06-15
影响因子：
2
通讯作者：
Morlock, Gertrud E.
中科院分区：
文献类型：
--
作者：
Chernetsova, Elena S.;Crawford, Elizabeth A.;Morlock, Gertrud E.
岩白菜是一种广泛用于草药的植物。它的化学成分很少被研究，也没有使用高分辨率质谱（HRMS）进行研究。它的酚类成分特别令人感兴趣，因为这些化合物在医药和化妆品中很受关注。ID-CUBE是一种简化的真实的时间直接分析（DART）离子源，适用于液体的快速质谱分析，无需复杂的样品制备，为研究此类植物提取物提供了一种新的方法。将ID-CUBE与高分辨率质谱仪耦合可提供浸提组分的鉴定。方法以黄酮类化合物柚皮素为模型溶液，优化质谱条件，并用于岩白菜绿色叶提取物中酚类化合物的鉴定。使用具有金属网格表面的OpenSpot样品卡将样品引入Obitrap质谱仪上的ID-CUBE离子源中。将样品作为5 μ L等份浸提液施加到卡的金属网格上。样品电离刺激的离子源在20？通过向金属网格施加电流以将分析物热解吸到来自ID-CUBE的亚稳态氦原子的气流中。结果提取物的质谱图中元素组成归属于丰富的离子。主要酚类成分由其[MH]离子确认。发现了36种其他标记离子，其中30%的元素组成是基于对草药提取物中发现的化合物的搜索。结论：ID-CUBEOrbitrap MS耦合允许快速准确地测定草药提取物中酚类成分（和其他化合物）的质量。通过使用其他结构解析方法，包括串联质谱法（MS/MS），可以提供更高的组分鉴定置信度，这将是未来研究的重点。版权所有（C）2012约翰威利父子有限公司
RATIONALE Bergenia crassifolia is a plant widely used in herbal medicine. Its chemical composition has been little studied, and no studies using high-resolution mass spectrometry (HRMS) have been performed. Its phenolic components are of particular interest, due to the interest in such compounds in medicine and cosmetics. The ID-CUBE, a simplified Direct Analysis in Real Time (DART) ion source, suitable for the fast MS analysis of liquids without complex sample preparation, offers a new method of studying extracts of such plant. Coupling the ID-CUBE with a high-resolution mass spectrometer can provide identification of extract components. METHODS Mass spectral conditions were optimized for model solutions of the flavonoid naringenin and used for the identification of phenolic compounds in green leaves extracts of Bergenia crassifolia. OpenSpot sample cards with a metal grid surface were used for sample introduction into the ID-CUBE ion source on an Obitrap mass spectrometer. The samples were applied as 5-mu L aliquots of the extract onto the metal grid of the card. Sample ionization was stimulated in the ion source within 20?s by applying an electric current to the metal grid to thermally desorb the analytes into the gas flow of metastable helium atoms from the ID-CUBE. RESULTS Elemental compositions were assigned to abundant ions in the mass spectra of the extracts. The major phenolic components were confirmed by their [MH] ions. Thirty-six other marker ions were found, and elemental compositions were suggested for 30% of them, based on a search for compounds found in herbal extracts. CONCLUSIONS The ID-CUBEOrbitrap MS coupling allowed the rapid accurate mass determination of the phenolic components (and other compounds) in herbal extracts. Higher confidence in component identification could be provided by using additional structural elucidation methods, including tandem mass spectrometry (MS/MS), and this will be the focus of future studies. Copyright (C) 2012 John Wiley & Sons, Ltd.