Rab 5 is required for the cellular entry of dengue and West Nile viruses
Rab 5 is required for the cellular entry of dengue and West Nile viruses
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DOI：
10.1128/jvi.02210-06
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发表时间：
2007-05-01
影响因子：
5.4
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
--
作者：
Krishnan, Manoj N.;Sukumaran, Bindu;Fikrig, Erol
登革热病毒和西尼罗河病毒进入细胞的机制尚未得到充分阐明。我们发现这两种病毒均通过网格蛋白依赖的内吞作用进入海拉细胞，并且需要液泡酸性pH值。分别对调节向早期或晚期核内体运输的GTP酶Rab 5或7进行抑制，结果表明Rab 5对登革热病毒和西尼罗河病毒的存活均至关重要。这些数据拓宽了我们对登革热病毒和西尼罗河病毒有效感染所需途径的理解，并可能有助于制定抗击疾病的新策略。
The mechanisms of cellular entry of dengue and West Nile viruses are not well characterized. We show that both these viruses enter HeLa cells by clathrin-dependent endocytosis and require vacuolar acidic pH. Inhibition of the GTPase Rab 5 or 7, which regulates transport to early or late endosomes, respectively, demonstrated that Rab 5 was essential for survival of both dengue and West Nile virus. These data broaden our understanding of the pathways required for productive dengue and West Nile virus infection and may facilitate new strategies for combating disease.