Immunohistochemical localization of growth factor-regulated channel (GRC) in human tissues
Immunohistochemical localization of growth factor-regulated channel (GRC) in human tissues
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DOI：
10.1507/endocrj.49.349
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发表时间：
2002-06-01
影响因子：
2
通讯作者：
Kojima, I
中科院分区：
文献类型：
--
作者：
Kowase, T;Nakazato, Y;Kojima, I
胰岛素样生长因子- 1激活钙渗透阳离子通道GRC（生长因子调节通道）。在本研究中，我们利用一种多克隆抗GRC抗体研究了GRC在人体组织中的免疫组织化学定位。胃、十二指肠、大肠、前列腺均可见免疫反应性GRC。在这些组织中，表达grc的细胞单独分布在上皮中，并共同表达嗜铬粒蛋白a（神经内分泌细胞的标志物）。GRC在胰管上皮、乳腺、腮腺和颌下腺中也有表达。肾小管上皮细胞和气管腺也被抗grc抗体染色。肺泡巨噬细胞表达GRC。在大脑中，小脑浦肯野细胞和脑膜蛛网膜细胞表达GRC。这些结果表明，GRC在特定组织的特定类型的细胞中表达，并且GRC可能调节这些细胞中的钙进入。
Insulin-like growth factor-I activates a calcium-permeable cation channel GRC (growth factor-regulated channel). In the present study, we investigated the immunohistochemical localization of GRC in human tissues using a polyclonal anti-GRC antibody. Immunoreactive GRC was detected in the stomach, duodenum, large intestine and prostate. In these tissues, GRC-expressing cells were distributed solitarily in the epithelium and coexpressed chromogranin-A, a marker of neuroendocrine cells. GRC was also expressed in the epithelium of the pancreatic duct, mammary gland, parotid gland, and submandibular gland. Epithelial cells of the renal tubule and the tracheal gland were also stained with anti-GRC antibody. In the lung, alveolar macrophages expressed GRC. In the brain, Purkinje cells of the cerebellum and arachnoid cells of the meningis expressed GRC. These results indicate that GRC is expressed in restricted types of cells in particular tissues, and that GRC may modulate calcium entry in these cells.