Nucleotide sequence surrounding the locus marker D21S246 on human chromosome 21.
Nucleotide sequence surrounding the locus marker D21S246 on human chromosome 21.
复制标题
人类 21 号染色体上基因座标记 D21S246 周围的核苷酸序列。
DOI：
10.1093/dnares/2.4.187
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Nomura,T
中科院分区：
文献类型：
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作者：
Yamazaki,M;Ono,A;Watanabe,K;Sasaki,K;Tashiro,H;Nomura,T
由于NotI酶的识别序列，迄今为止发现的大多数人NotI连接克隆被认为是来自CpG岛，并且据报道CpG岛位于基因的5′区域周围。作为初步研究，我们测定了含有源自aNotI连接克隆的标记D21 S246的人粘粒克隆（LL 21 NC 02 Q7 A10）的完整核苷酸序列（41，924 bp）。作为序列分析的结果，我们成功地映射并揭示了基因组基因结构KIAA 0002先前报告的cDNA克隆。该基因由15个外显子组成，存在于人类染色体21q21.3-q22.1上的D21 S246位点。这些结果表明，基因组标记锚定DNA测序是一个有用的方法，为人类基因组计划。
Most ofthe humanNotI linking clones identified to date are considered to be derived from CpG islands because ofthe recognition sequence of this enzyme, and CpG islands have been reported to be located around the 5′ regions of genes. As a pilot study, we determined the complete nucleotide sequence (41,924 bp) of a human cosmid clone (LL21NC02Q7A10) containing the marker D21S246 originating from aNotI linking clone. As a result of sequence analysis, we successfully mapped and revealed the genomic gene structure for KIAA0002 previously reported as a cDNA clone. This gene consists of 15 exons and was shown to exist at the D21S246 locus on human chromosome 21q21.3–q22.1. These results demonstrated that genomic marker-anchored DNA sequencing is a useful approach for the human genome project.