ZINC OXIDE NANOPARTICLES TOXICITY TO DAPHNIA MAGNA: SIZE-DEPENDENT EFFECTS AND DISSOLUTION
ZINC OXIDE NANOPARTICLES TOXICITY TO DAPHNIA MAGNA: SIZE-DEPENDENT EFFECTS AND DISSOLUTION
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DOI：
10.1002/etc.2413
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发表时间：
2014-01-01
影响因子：
4.1
通讯作者：
Loureiro, Susana
中科院分区：
文献类型：
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作者：
Lopes, Silvia;Ribeiro, Fabianne;Loureiro, Susana
随着氧化锌纳米颗粒（ZnO-NP）和其他金属氧化物的产量呈指数级增长，研究此类纳米颗粒的潜在环境和健康影响非常重要。纳米颗粒的特性（例如尺寸、溶解速率）在不同的水介质中可能会发生变化，它们的表征对于得出有关毒性结果的结论至关重要。因此，利用水生模式生物 Daphnia magna 研究了 2 种不同粒径（30 nm 和 80-100 nm）的 ZnO-NP 的影响，然后将这些效果与 ZnO 微米级颗粒（> 200 nm）和离子对应物（ZnCl2 形式）对固定、摄食抑制和繁殖终点的影响进行比较。固定化的 48 小时半数致死浓度 (LC50) 范围为离子锌的 0.76mg Zn L-1 和 80nm 至 100nm ZnO-NP 的 1.32mg Zn L-1。对于长期暴露，在锌暴露中，生殖输出也受到类似的损害，并且可能主要由锌离子形式驱动。所用浓度显示48小时后完全溶解。另一方面，与离子锌相比，30nm ZnO-NPs 的取食活动更受影响，表明颗粒形式在 D. magna 的取食抑制中也发挥着重要作用。人们发现水蚤测试介质中的溶解度和粒径对于得出毒性结论至关重要。因此，它们可能被认为对于评估纳米颗粒的毒性和命运至关重要。环境毒理学化学 2014；33：190-198。 (c) 2013 年 SETAC
As the production of zinc oxide nanoparticles (ZnO-NPs) and other metal oxides is exponentially increasing, it is important to investigate potential environmental and health impacts of such nanoparticles. Nanoparticles' properties (e.g., size, dissolution rate) may change in different water media, and their characterization is essential to derive conclusions about toxicity results. Therefore, an aquatic model organism, Daphnia magna, was used to investigate the effect of ZnO-NPs with 2 different particle sizes (30nm and 80-100nm) and then compare these effects with ZnO microsized particles (>200nm) and the ionic counterpart (in the form of ZnCl2) on immobilization, feeding inhibition, and reproduction endpoints. The 48-h median lethal concentration (LC50) for immobilization ranged between 0.76mg Zn L-1 for the ionic zinc and 1.32mg Zn L-1 for ZnO-NPs of 80nm to 100nm. For the chronic exposures, the reproduction output was impaired similarly among zinc exposures and possibly driven mainly by the zinc ionic form. The concentrations used showed a total dissolution after 48h. On the other hand, feeding activity was more affected by the 30nm ZnO-NPs than by the ionic zinc, showing that the particulate form was also playing an important role in the feeding inhibition of D. magna. Dissolution and particle size in the daphnia test media were found to be essential to derive conclusions on toxicity. Therefore, they can possibly be considered critical for evaluating nanoparticles' toxicity and fate. Environ Toxicol Chem 2014;33:190-198. (c) 2013 SETAC