Immunological Detection of Phosphorylation
Immunological Detection of Phosphorylation
复制标题
磷酸化的免疫学检测
DOI：
10.1002/0471143030.cb1402s00
复制
发表时间：
1998
影响因子：
--
通讯作者：
R. Seger
中科院分区：
文献类型：
--
作者：
Zhong Yao;R. Seger
Incorporation of phosphates into serine, threonine, and tyrosine acceptors in proteins is a common mechanism for regulating protein function. This unit presents protocols that use specific anti‐phosphoamino acid (PAA) and anti‐phosphoprotein antibodies to detect protein phosphorylation and protein kinase activity. Immunoblotting to detect protein phosphorylation using either anti‐PAA or anti‐phosphoprotein antibodies. This is a convenient method that usually yields impressive results. Phosphorylation can also be detected by immunoprecipitation followed by immunoblot analysis or by immunofluorescent staining; these methods are typically more complicated and time consuming. All three methods have been successfully used to detect protein phosphorylation with a wide variety of antibodies and most phosphorylated proteins.
影响因子：
56.9
作者：
GINTY, DD;KORNHAUSER, JM;GREENBERG, ME
通讯作者：
GREENBERG, ME
在完整细胞对胰岛素的初始反应期间，胰岛素受体酪氨酸磷酸化占主导地位。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pang,DT;Sharma,BR;Shafer,JA;White,MF;Kahn,CR
通讯作者：
Kahn,CR