Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Biomonitoring Cooked Meat Carcinogens and Their Metabolites in Human Urine
Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Biomonitoring Cooked Meat Carcinogens and Their Metabolites in Human Urine
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DOI：
10.1021/ac102918b
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发表时间：
2011-02-01
影响因子：
7.4
通讯作者：
Turesky, Robert J.
中科院分区：
文献类型：
--
作者：
Gu, Dan;Raymundo, Melissa M.;Turesky, Robert J.
本文同时测定了杂食动物尿液中万亿分之一水平的熟肉致癌物2-氨基-1-甲基-6-苯基咪唑[4，5-B]吡啶（PhIP）、2-氨基-3，8-二甲基咪唑[4，5-f]喹喔啉（MeIQ 2x）及其主要代谢产物，通过具有Michrom Advance CaptiveSpray源和三级四极杆质谱仪的超高效液相色谱法（UPLC）测定。在选定的反应监测模式下进行定量。UPLC方法比我们早期的毛细管HPLC方法更加快速和灵敏：UPLC方法的占空比为19 min，而毛细管HPLC为57 min。采用3名受试者在4个不同日期的尿样评价了UPLC测定的性能。PhIP、MeIQx及其代谢产物估计值的日内和日间精密度（报告为变异系数）为
The cooked meat carcinogens 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQ2x), and their principal metabolites produced by cytochrome P450 and/or uridine diphosphate glucuronosyl transferases were simultaneously measured at the parts per trillion level in urine of omnivores, by ultraperformance liquid chromatography (UPLC) with a Michrom Advance CaptiveSpray source and a triple stage quadrupole mass spectrometer. Quantitation was performed in the selected reaction monitoring mode. The UPLC method is much more rapid and sensitive than our earlier capillary HPLC method: the duty Cycle of the UPLC method is 19 min compared to 57 min for capillary HPLC. The performance of the UPLC assay was evaluated with urine samples from three subjects over 4 different days. The intraday and interday precisions of the estimates of PhIP, MeIQx, and their metabolites, reported as the coefficients of variation, were