A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells
A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells
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DOI：
10.1073/pnas.94.10.5261
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发表时间：
1997-05-13
影响因子：
11.1
通讯作者：
Coopers, MD
中科院分区：
文献类型：
--
作者：
Kubagawa, H;Burrows, PD;Coopers, MD
用人源Fc α受体探针鉴定了小鼠IG基因超家族的新成员。从小鼠脾文库中分离的cDNA序列分析预测了成对的Ig样受体，命名为PIR-A和PIR-B。这两种I型跨膜蛋白具有类似的六个Ig样环的胞外域，但具有不同的跨膜区和胞质区，预测的PIR-A蛋白具有短的胞质尾区和跨膜区中的带电Arg残基，通过与FccuR相对物类比，表明与另外的跨膜蛋白缔合以形成信号转导单元的潜力。相反，PIR-B蛋白具有不带电荷的跨膜区和含有四个潜在的基于免疫受体酪氨酸的抑制基序的长胞质尾。这些特征是由相关的杀伤抑制受体共享的，PIR-A蛋白似乎是高度可变的，因为预测的肽序列对于7个随机选择的PIR-A克隆是不同的，而PIR-B cDNA克隆是不变的，用PIR-B和PIR-A特异性探针的Southern印迹分析表明只有一个PIR-B基因和多个PIR-A基因。PIR-A和PIR-B基因在B淋巴细胞和髓系细胞中表达，其中两者同时表达。高度保守的PIR-A和PIR-B基因的特征及其协同细胞表达表明在体液、炎症和过敏反应中的潜在调节作用。
An Fc alpha receptor probe of human origin was used to identify novel members of the Ig gene superfamily in mice, Paired Ig-like receptors, named PIR-A and PIR-B, are predicted from sequence analysis of the cDNAs isolated from a mouse splenic library, Both type I transmembrane proteins possess similar ectodomains with six Ig-like loops, but have different transmembrane and cytoplasmic regions, The predicted PIR-A protein has a short cytoplasmic tail and a charged Arg residue in the transmembrane region that, by analogy with the FccuR relative, suggests the potential for association with an additional transmembrane protein to form a signal transducing unit. In contrast, the PIR-B protein has an uncharged transmembrane region and a long cytoplasmic tail containing four potential immunoreceptor tyrosine-based inhibitory motifs. These features are shared by the related killer inhibitory receptors, PIR-A proteins appear to be highly variable, in that predicted peptide sequences differ for seven randomly selected PIR-A clones, whereas PIR-B cDNA clones are invariant, Southern blot analysis with PIR-B and PIR-A-specific probes suggests only one PIR-B gene and multiple PIR-A genes. The PIR-A and PIR-B genes are expressed in B lymphocytes and myeloid lineage cells, wherein both are expressed simultaneously, The characteristics of the highly-conserved PIR-A and PIR-B genes and their coordinate cellular expression suggest a potential regulatory role in humoral, inflammatory, and allergic responses.