Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.
Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.
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单脂质提取：胆固醇在液体有序相和液体无序相中的锚定强度。
DOI：
10.1016/j.bpj.2014.07.018
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发表时间：
2014
影响因子：
3.4
通讯作者：
T. Hugel
中科院分区：
文献类型：
--
作者：
F. Stetter;Lukasz Cwiklik;P. Jungwirth;T. Hugel
胆固醇对于支撑脂质双层中微区的形成是重要的，并且在液体有序相中富集。为了了解导致这种富集的相互作用，我们开发了一种基于AFM的单脂质提取（SLX）方法，使我们能够确定胆固醇在相分离的脂质膜的两相中的锚定强度。正如预期的那样，从液体有序相中提取单个胆固醇分子所需的力显著高于从液体无序相中提取它所需的力。有趣的是，贝尔模型的应用显示了与胆固醇分子的头部和全长相关的两个能量势垒。完全提取的寿命分别为90 s和11 s的液体有序和液体无序相。同样的实验的分子动力学模拟显示出相似的力分布，并表明胆固醇在液体有序相的稳定化主要是由于非极性接触。
Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquid-ordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approach that enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higher than for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are 90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-ordered phase is mainly due to nonpolar contacts.
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DOI：
10.1021/jp808412x
发表时间：
2009
期刊：
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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