Apoptosis-inducing galactolipids from a cultured, marine diatom, Phaeodactylum tricornutum
Apoptosis-inducing galactolipids from a cultured, marine diatom, Phaeodactylum tricornutum
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DOI：
10.1021/np800124k
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发表时间：
2008-07-01
影响因子：
5.1
通讯作者：
Falkowski, Paul
中科院分区：
文献类型：
--
作者：
Andrianasolo, Eric H.;Haramaty, Liti;Falkowski, Paul
两个单半乳糖基二酰基甘油，1和2，分离自海洋硅藻Phaeodactylum tricornutum，使用专利的ApopScreen细胞为基础的筛选，诱导细胞凋亡，潜在的抗癌化合物。使用NMR、质谱和化学降解的组合测定半乳糖脂的分子结构。使用基于具有差异的、确定的细胞凋亡能力的基因工程哺乳动物细胞系的特异性细胞凋亡诱导测定来确认生物活性。半乳糖脂在微摩尔浓度下诱导细胞凋亡。这是首次报道半乳糖脂诱导细胞凋亡。
Two monogalactosyl diacylglycerols, 1 and 2, were isolated from the marine diatom Phaeodactylum tricornutum, using the patented ApopScreen cell-based screen for apoptosis-inducing, potential anticancer compounds. The molecular structures of the galactolipids were determined using a combination of NMR, mass spectrometry, and chemical degradation. The bioactivities were confirmed using a specific apoptosis induction assay based on genetically engineered mammalian cell lines with differential, defined capacities for apoptosis. The galactolipids induce apoptosis in micromolar concentrations. This is the first report of apoptosis induction by galactolipids.