Involvement of clip-domain serine protease in the anti-Vibrio immune response of abalone (Haliotis discus hannai)- Molecular cloning, characterization and functional analysis
Involvement of clip-domain serine protease in the anti-Vibrio immune response of abalone (Haliotis discus hannai)- Molecular cloning, characterization and functional analysis
复制标题
夹域丝氨酸蛋白酶参与鲍鱼抗弧菌免疫反应 - 分子克隆、表征和功能分析
DOI：
10.1016/j.fsi.2017.10.062
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Cao, Min-Jie
中科院分区：
文献类型：
--
作者：
Hu, Jian-Jian;Chen, Yu-Lei;Cao, Min-Jie
副溶血性弧菌（Vibrio parahemolyticus，V. parahemolyticus）是我国沿海重要经济贝类鲍的主要致病菌。鲍鱼抗病原体感染的天然免疫系统目前还知之甚少。剪切结构域丝氨酸蛋白酶（Clip-domain serine proteases，cSPs）在无脊椎动物的免疫防御中发挥着重要作用。本研究从皱纹盘鲍（Haliotis discus hannai）中克隆了一个cSP（Hdh-cSP）。结果表明，Hdh-cSP在鲍鱼多种组织中广泛表达，其中在免疫样器官肝胰腺中表达量最高。副溶血性弧菌感染诱导了Hdh-cSP的显著升高的表达，以及更好表征的先天免疫组分基因，包括Rel/NF-κ B、同种异体移植物炎性因子（ALInFa）、巨噬细胞表达蛋白（MEP）和半胱天冬酶-8。重要的是，Hdh-cSP的沉默降低了这些基因的表达，表明Hdh-cSP是副溶血性弧菌感染中的上游调控因子。进一步分析表明，Hdh-cSP的转录被敲低后，血细胞的凋亡受到抑制，提示Hdh-cSP通过调控caspase 8的表达参与了副溶血弧菌感染后的细胞凋亡。因此，我们的研究证实了cSP在鲍鱼抗副溶血性弧菌感染的天然免疫中的重要作用。
Vibrio parahemolyticus (V. parahemolyticus) is a major pathogen for abalone, an important economical shellfish in coastal area of China. There is little known about the abalone innate immune system against pathogen infection. Clip-domain serine proteases (cSPs) are increasingly recognized to play important roles in host inunune defense in invertebrates. In this study, we cloned a cSP (Hdh-cSP) from abalone (Haliotis discus hannai). We found out that Hdh-cSP was widely expressed in multiple tissues of abalone, with highest level in the immune-like organ, hepatopancreas. V. parahemolyticus infection induced significantly elevated expression of Hdh-cSP in addition to better-characterized innate immune component genes including Rel/NF-kappa B, allograft inflammatory factor (ALInFa), macrophage expressed protein (MEP) and caspase-8. Importantly, the silencing of Hdh-cSP reduced the expression of these genes, suggesting that Hdh-cSP was an upstream regulatory factor in V. parahemolyticus infection. Further analysis showed that apoptosis of hemocytes was inhibited when the transcription of Hdh-cSP was knocked down, suggesting that Hdh-cSP participated in cell apoptosis by regulation of caspase 8 expression in V. parahemolyticus infection. Therefore, our study established an important role of cSP in the innate immunity against V. parahemolyticus infection in abalone.