Novel bacterial diversity is enriched with chloroperoxidase-reacted organic matter under anaerobic conditions.
Novel bacterial diversity is enriched with chloroperoxidase-reacted organic matter under anaerobic conditions.
复制标题
在厌氧条件下，氯过氧化物酶反应的有机物丰富了新的细菌多样性。
DOI：
10.1093/femsec/fiy050
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发表时间：
2018
影响因子：
4.2
通讯作者：
Krzmarzick, Mark James
中科院分区：
文献类型：
--
作者：
Lim, Ming Li;Brooks, Matthew DeWayne;Boothe, Melissa Anne;Krzmarzick, Mark James
真菌氯过氧化物酶（CPOs）是一类在土壤中产生天然有机氯化物的酶。这些有机氯化物的微生物降解尚不清楚，但对生物修复、微生物生态学和天然氯和碳循环具有重要意义。本研究采用基于illumina的16S rRNA基因测序和实时定量PCR （qPCR）技术，对有利于氯化作用（CPO- om）条件下有机物与CPO反应后富集的细菌群落进行了表征。在接种了小溪底泥并添加了CPO-OM的三次培养基中，总共富集了17个细菌群。这些细菌群既没有被未反应的有机物提取物的修饰物（有或没有H2O2诱导的氧化应激）富集，也没有在非氯化条件下与CPO反应的有机物修饰物富集。其中，只有两个具有已知的有机卤化物呼吸细菌的代表属-脱卤单胞菌和脱卤杆菌。acetobacteria属也被发现富集，但其他14组富集细菌目前没有任何与系统发育密切相关的分离菌株。该研究强调了目前对天然有机氯化物转化的微生物学理解的空白，并表明CPO-OM可用于分离和培养新细菌属的菌株。
Fungal chloroperoxidases (CPOs) are one class of enzymes that produce natural organochlorides in soils. The microbial degradation of these organochlorides is not well known, though has implications for bioremediation, microbial ecology and natural chlorine and carbon cycling. In this study, Illumina-based 16S rRNA gene sequencing and real-time quantitative PCR (qPCR) was used to characterize the bacterial community enriched from an amendment of organic matter reacted with CPO under conditions conducive towards chlorination (CPO-OM). In total, 17 bacterial groups were enriched in triplicate microcosms inoculated with creek sediment and amended with CPO-OM. These bacterial groups were neither enriched with amendments of non-reacted organic matter extract, with or without oxidative stress induced by H2O2, nor with amendments of organic matter reacted with CPO under non-chlorinating conditions. Of these, only two represented genera with known organohalide respiring bacteria—DehalogenimonasandDehalobacter. The genusAcetobacteriumwas also found to be enriched but the other 14 groups of enriched bacteria do not currently have any close phylogenetically related isolates. This study highlights a gap in the current understanding of the microbiology involved in natural organochloride turnover and suggests that CPO-OM could be used for isolating and culturing strains from novel bacteria genera.