A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells
A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells
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DOI：
10.1186/s41240-019-0126-3
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发表时间：
2019-05-31
影响因子：
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通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
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作者：
Wang, Lei;Cui, Yong Ri;Jeon, You-Jin
背景资料：在本研究中，研究了一种海洋来源的混合物的皮肤美白效果，该混合物包括裙带菜的富含岩藻多糖的提取物（UPEF）、腔海桑的富含间苯甲素的提取物（ECE）和来自海鞘皮肤的糖胺聚糖（GAG）。通过测量α-黑素细胞中蘑菇酪氨酸酶和黑素合成的抑制来评价混合物及其组分的美白效果。结果：各组分单独作用时对蘑菇酪氨酸酶有明显的抑制作用，且呈剂量依赖性;在α-MSH刺激的B16 F10细胞中，各组分对黑色素合成有抑制作用，并具有细胞毒性。然而，UPEF、ECE和GAG组合的美白效果大于单独使用每种成分的美白效果。以4：5：1的比例混合（UEG-451）显示出最强的活性，且无细胞毒性。进一步的研究表明，UEG-451通过抑制MITF的表达，下调酪氨酸酶和酪氨酸酶相关蛋白如TRP-1和TRP-2，从而抑制α-MSH刺激的B16 F10细胞黑素生成。这些结果表明，将不同组分以最佳比例混合可能是提高其生物活性和降低毒性的有效方法，UEG-451具有很强的增白作用，可用于化妆品行业。
Background: In the present study, the skin-whitening effects of a marine-sourced mixture that includes a fucoidan-rich extract of Undaria pinnatifida (UPEF), a phlorotannin-rich extract of Ecklonia cava (ECE), and glycosaminoglycans (GAGs) from sea squirt skin were investigated.Methods: The whitening effects of the mixture and its components were evaluated by measuring the inhibition of mushroom tyrosinase and melanin synthesis in alpha-melanocyte-stimulating hormone (alpha-MSH)-stimulated B16F10 melanoma cells.Results: Each component alone markedly inhibited mushroom tyrosinase in a dose-dependent manner, and in -MSH-stimulated B16F10 cells, they inhibited melanin synthesis and were cytotoxic. However, the whitening effects of UPEF, ECE, and GAGs in combination were greater than those of each component alone. A mixture in the ratio of 4:5:1 (UEG-451) showed the strongest activity without cytotoxicity. Further study suggested that UEG-451 inhibits alpha-MSH-stimulated melanogenesis in B16F10 cells by downregulating tyrosinase and tyrosinase-related proteins, such as TRP-1 and TRP-2, via the inhibition of MITF expression.Conclusions: These results suggest that mixing the different components at optimum ratios might be an effective way to improve their bioactivities and reduce toxicity and that UEG-451 possesses strong whitening effects that could be used in the cosmetic industry.