Molecular cartography of globular proteins with application to antigenic sites.
Molecular cartography of globular proteins with application to antigenic sites.
复制标题
球状蛋白的分子制图及其抗原位点的应用。
DOI：
10.1002/bip.360250509
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Rose,GD
中科院分区：
文献类型：
--
作者：
Fanning,DW;Smith,JA;Rose,GD
介绍了一种定量蛋白质表面拓扑结构的方法，分子制图法。该方法适用于抗原决定簇的问题，它是用来检查报告的肌红蛋白和溶菌酶的表面上的抗原区域的局部和全局地形。在从文献中提取并详细研究的9个抗原位点中，在表面的其余区域中也没有发现的位点中没有发现局部性质。然而，发现抗原位点和表面区域之间存在很强的相关性。这一发现表明，蛋白质表面的全局暴露可能在决定蛋白质的抗原结构中起主要作用。分子制图学可能在蛋白质-蛋白质相互作用的其他情况下有用，例如蛋白水解酶与其底物之间的相互作用。
A method, molecular cartography, is introduced as a way to quantitate the topographic structure of a protein surface. The method is applied to the problem of antigenic determinants, and it is used to examine local and global topography of reported antigenic regions on the surface of myoglobin and lysozyme. In nine antigenic sites taken from the literature and studied in detail, no local property was found in sites that was not also found in remaining regions of the surface. However, a strong correlation was found between antigenic sites and regions of the surface that are globally exposed. This finding suggests that global exposure of the protein surface may play a primary role in determining the antigenic structure of the protein. Molecular cartography may be useful in other instances of protein–protein interactions such as those between proteolytic enzymes and their substrates.