Expression of cytochrome P450 enzymes in hepatic organoid reconstructed by rat small hepatocytes
Expression of cytochrome P450 enzymes in hepatic organoid reconstructed by rat small hepatocytes
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DOI：
10.1111/j.1440-1746.2005.03804.x
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发表时间：
2005-06
影响因子：
4.1
通讯作者：
S. Miyamoto;K. Hirata;Shinichi Sugimoto;K. Harada;T. Mitaka
中科院分区：
文献类型：
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作者：
S. Miyamoto;K. Hirata;Shinichi Sugimoto;K. Harada;T. Mitaka
背景和目的：小肝细胞（SH），这是肝祖细胞，从成年大鼠肝脏分离。菌落中的SH有时会从小到大，从扁平到上升/堆积。SH的形态学变化可能与肝脏成熟有关。细胞色素P450（CYP 450）是药物代谢酶，其表达是肝脏分化功能之一。然而，众所周知，在培养的肝细胞中，重新表达和维持α-淀粉酶活性非常困难。我们研究了在长期培养的SH中的β-淀粉样蛋白的表达和酶活性。
Background and Aims: Small hepatocytes (SH), which are hepatic progenitor cells, were isolated from an adult rat liver. SH in a colony sometimes change their shape from small to large and from flat to rising/piled‐up. The morphological changes of SH may be correlated with hepatic maturation. Cytochrome P450s (CYP) are drug‐metabolizing enzymes and the expression is one of hepatic differentiated functions. However, it is well known that the re‐expression and maintenance of CYP activity are very difficult in cultured hepatocytes. We investigated the expression of CYP and the enzymatic activities in long‐term cultured SH.