Survival of neural progenitor cells from the subventricular zone of the adult rat after transplantation into the host spinal cord of the same strain of adult rat
Survival of neural progenitor cells from the subventricular zone of the adult rat after transplantation into the host spinal cord of the same strain of adult rat
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DOI：
10.1111/j.1447-073x.2005.00128.x
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发表时间：
2005-12-01
影响因子：
1.2
通讯作者：
Ide, Chizuka
中科院分区：
文献类型：
--
作者：
Mligiliche, Nurru Lameck;Xu, Yi;Ide, Chizuka
哺乳动物前脑侧脑室的室下区（SVZ）是神经祖细胞（NPC）在成人大脑中持续存在的主要部位。鼻咽癌位于室管膜细胞下方，具有自我更新和不断产生神经元和神经胶质细胞的能力。我们以前已经证明，神经球可以从死亡的成年大鼠的大脑中获得，并且神经球细胞在移植到脊髓后存活。在本研究中，我们研究了活体成年大鼠的新鲜NPC移植到同一品系的成年大鼠脊髓后能否存活并整合到宿主组织中。我们用表达转基因绿色荧光蛋白（GFP）的大鼠作为供体来鉴定移植的npc。从成年gfp大鼠侧脑室纹状体壁获得SVZ组织，并在颈椎水平移植到脊髓病变处。移植后2 ~ 3周，NPC在距植入部位0.5 ~ 1 mm的宿主组织中迁移，并融入宿主脊髓白质中。存活的NPC表现出星形胶质细胞（胶质纤维酸性蛋白）的免疫组织化学表型，但没有神经元（β -微管蛋白III）或少突胶质细胞（Rip; Hybridoma Bank, Iowa City， A， USA）。因此，来自成年大鼠SVZ的NPC可以存活并分化成至少星形胶质细胞，然后在移植到成年大鼠脊髓病变后可以整合到宿主组织中。
The subventricular zone (SVZ) of the lateral ventricle of the mammalian forebrain is the major site in which neural progenitor cells (NPC) persist in the adult brain. The NPC are located beneath ventricular ependymal cells and have the capacity to self-renew and continuously produce neurons and glial cells. We have shown previously that neurospheres can be obtained from the brain of deceased adult rats and that neurosphere cells survive after transplantation into the spinal cord. In the present study, we investigated whether fresh NPC from living adult rats can survive and be integrated into host tissues after transplantation into the adult rat spinal cord of the same strain. We used rats expressing transgenic green fluorescent protein (GFP) as a donor to identify the transplanted NPCs. The SVZ tissues were obtained from the striatal wall of the lateral ventricle of adult GFP-rats and Were grafted into lesions of the spinal cord at the cervical level. Two to 3 weeks after grafting, NPC migrated through the host tissue 0.5-1 mm away from the implantation site, and were integrated into the white matter of the host spinal cord. Surviving NPC exhibited immunohistochemical phenotypes of astrocytes (glial fibrillary acidic protein), but not for neurons (beta-tubulin III) or oligodendrocytes (Rip; Hybridoma Bank, Iowa City, A, USA). Thus, NPC from the SVZ of adult rats can survive and differentiate into at least astrocytes, which can then be integrated into host tissue after transplantation into spinal cord lesions in the adult rat.