Metabolic alterations in the sera of Chinese patients with mild persistent asthma: a GC-MS-based metabolomics analysis
Metabolic alterations in the sera of Chinese patients with mild persistent asthma: a GC-MS-based metabolomics analysis
复制标题
中国轻度持续性哮喘患者血清代谢变化：基于 GC-MS 的代谢组学分析
DOI：
10.1038/aps.2015.102
复制
发表时间：
2015-11-01
影响因子：
8.2
通讯作者：
Yao, Wan-zhen
中科院分区：
文献类型：
--
作者：
Chang, Chun;Guo, Zhi-guo;Yao, Wan-zhen
目的：目的：研究中国轻度持续性哮喘患者血清中特异性代谢组学特征，探索潜在的代谢生物标志物。方法：17例中国轻度持续性哮喘患者和年龄、性别匹配的健康对照者入选。结果：哮喘组与对照组间存在明显的组间差异，哮喘组与对照组间差异无统计学意义。鉴别了一系列差异代谢物和几种改变最多的代谢途径。琥珀酸（三羧酸循环中的中间产物）和肌苷的水平在哮喘患者中高度上调，表明在哮喘急性发作和缺氧应激期间呼吸的努力更大。还鉴定了其他差异代谢物，如3，4-二羟基苯甲酸和苯丙氨酸。此外，差异代谢产物具有较高的ROC曲线下面积（AUC）值，提示其具有良好的临床预测哮喘的能力。结论：轻度持续性哮喘患者血清中代谢活性明显改变。这些数据可能有助于识别新的哮喘生物标志物和治疗靶点。
Aim: To character the specific metabolomics profiles in the sera of Chinese patients with mild persistent asthma and to explore potential metabolic biomarkers.Methods: Seventeen Chinese patients with mild persistent asthma and age-and sex-matched healthy controls were enrolled. Serum samples were collected, and serum metabolites were analyzed using GC-MS coupled with a series of multivariate statistical analyses.Results: Clear intergroup separations existed between the asthmatic patients and control subjects. A list of differential metabolites and several top altered metabolic pathways were identified. The levels of succinate (an intermediate in tricarboxylic acid cycle) and inosine were highly upregulated in the asthmatic patients, suggesting a greater effort to breathe during exacerbation and hypoxic stress due to asthma. Other differential metabolites, such as 3,4-dihydroxybenzoic acid and phenylalanine, were also identified. Furthermore, the differential metabolites possessed higher values of area under the ROC curve (AUC), suggesting an excellent clinical ability for the prediction of asthma.Conclusion: Metabolic activity is significantly altered in the sera of Chinese patients with mild persistent asthma. The data might be helpful for identifying novel biomarkers and therapeutic targets for asthma.