MTERF4 Regulates Translation by Targeting the Methyltransferase NSUN4 to the Mammalian Mitochondrial Ribosome
MTERF4 Regulates Translation by Targeting the Methyltransferase NSUN4 to the Mammalian Mitochondrial Ribosome
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DOI：
10.1016/j.cmet.2011.04.002
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发表时间：
2011-05-04
期刊：
影响因子：
29
通讯作者：
Larsson, Nils-Goran
中科院分区：
文献类型：
--
作者：
Camara, Yolanda;Asin-Cayuela, Jorge;Larsson, Nils-Goran
精确控制线粒体！DNA基因表达对哺乳动物氧化磷酸化能力的调节至关重要。MTERF蛋白家族在这一过程中起着关键作用，其成员参与了转录起始和位点特异性转录终止的调控。我们现在证明，这个家庭的成员，MTERF 4，直接控制线粒体核糖体的生物合成和翻译。MTERF 4与核糖体RNA甲基转移酶NSUN 4形成化学计量复合物，并且是将该因子募集到核糖体大亚基所必需的。MTERF 4的缺失导致核糖体组装缺陷和翻译急剧减少。因此，我们的研究结果表明，MTERF 4是一个重要的哺乳动物线粒体翻译调节。
Precise control of mitochondria! DNA gene expression is critical for regulation of oxidative phosphorylation capacity in mammals. The MTERF protein family plays a key role in this process, and its members have been implicated in regulation of transcription initiation and site-specific transcription termination. We now demonstrate that a member of this family, MTERF4, directly controls mitochondrial ribosomal biogenesis and translation. MTERF4 forms a stoichiometric complex with the ribosomal RNA methyltransferase NSUN4 and is necessary for recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation. Our results thus show that MTERF4 is an important regulator of translation in mammalian mitochondria.