A computational index derived from whole-genome copy number analysis is a novel tool for prognosis in early stage lung squamous cell carcinoma
A computational index derived from whole-genome copy number analysis is a novel tool for prognosis in early stage lung squamous cell carcinoma
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DOI：
10.1016/j.ygeno.2011.10.006
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发表时间：
2012-01-01
期刊：
影响因子：
4.4
通讯作者：
Wood, Henry M.
中科院分区：
文献类型：
--
作者：
Belvedere, Ornella;Berri, Stefano;Wood, Henry M.
肺鳞状细胞癌的显著之处在于在单个肿瘤中检测到相同染色体异常的程度。我们已经使用低覆盖率的下一代测序来产生一系列89个非小细胞肺turnout的高分辨率拷贝数核型图，特别是鳞状细胞亚型。因为这种方法能够从福尔马林固定的石蜡包埋材料中创建核型图，所以我们能够使用可获得存活数据的存档存储样品，并将频繁发生的拷贝数变化与疾病结果相关联。没有单一区域的基因组变化与生存率有显著相关性。然而，采用全基因组方法，我们设计了一种与总基因组损伤相关的算法，特别是整个基因组中拷贝数状态的相对比率。该算法产生了一个新的指数，这是一个独立的早期肺鳞状细胞癌的预后指标。(C)2011 Elsevier Inc. All rights reserved.
Squamous cell carcinoma of the lung is remarkable for the extent to which the same chromosomal abnormalities are detected in individual tumours. We have used next generation sequencing at low coverage to produce high resolution copy number karyograms of a series of 89 non-small cell lung turnouts specifically of the squamous cell subtype. Because this methodology is able to create karyograms from formalin-fixed paraffin-embedded material, we were able to use archival stored samples for which survival data were available and correlate frequently occurring copy number changes with disease outcome. No single region of genomic change showed significant correlation with survival. However, adopting a whole-genome approach, we devised an algorithm that relates to total genomic damage, specifically the relative ratios of copy number states across the genome. This algorithm generated a novel index, which is an independent prognostic indicator in early stage squamous cell carcinoma of the lung. (C) 2011 Elsevier Inc. All rights reserved.