Diversity and phylogeny of Baltic Sea picocyanobacteria inferred from their ITS and phycobiliprotein operons
Diversity and phylogeny of Baltic Sea picocyanobacteria inferred from their ITS and phycobiliprotein operons
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DOI：
10.1111/j.1462-2920.2007.01442.x
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发表时间：
2008-01-01
影响因子：
5.1
通讯作者：
Stal, Lucas J.
中科院分区：
文献类型：
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作者：
Haverkamp, Thomas;Acinas, Silvia G.;Stal, Lucas J.
聚藻球菌属的Picocyanobacteria跨越了一系列不同的颜色，从富含藻红蛋白（PE）的红色菌株到富含藻蓝蛋白（PC）的绿色菌株。在这里，我们表明，红色和绿色的picocyanobacteria共存在波罗的海是普遍的。利用16S rRNA-ITS、红色PE色素的cpeBA操纵子和绿色PC色素的cpcBA操纵子3个不同的基因，分析了红色和绿色picocyanobacteria的多样性和系统发育。209个克隆的测序结果表明，波罗的海picocyanobacteria表现出高水平的微多样性。波罗的海克隆文库cpcBA和cpeBA操纵子的部分核苷酸序列揭示了两个不同的系统发育分支：一个分支主要含有培养的富含pc的picocyanobacteria的序列，而另一个分支只含有培养的富含pe的菌株的序列。含有富含pe的聚藻球菌属的第三支藻胆碱（PUB）不含波罗的海克隆文库中的序列。这些发现不同于先前发表的基于16S rRNA基因分析的系统发育。我们的数据表明，就其色素沉着而言，聚球菌属代表了三个不同的谱系，在水下光谱中占据不同的生态位。来自不同谱系的菌株可以在与其光吸收光谱重叠的光环境中共存。
Picocyanobacteria of the genus Synechococcus span a range of different colours, from red strains rich in phycoerythrin (PE) to green strains rich in phycocyanin (PC). Here, we show that coexistence of red and green picocyanobacteria in the Baltic Sea is widespread. The diversity and phylogeny of red and green picocyanobacteria was analysed using three different genes: 16S rRNA-ITS, the cpeBA operon of the red PE pigment and the cpcBA operon of the green PC pigment. Sequencing of 209 clones showed that Baltic Sea picocyanobacteria exhibit high levels of microdiversity. The partial nucleotide sequences of the cpcBA and cpeBA operons from the clone libraries of the Baltic Sea revealed two distinct phylogenetic clades: one clade containing mainly sequences from cultured PC-rich picocyanobacteria, while the other contains only sequences from cultivated PE-rich strains. A third clade of phycourobilin (PUB) containing strains of PE-rich Synechococcus spp. did not contain sequences from the Baltic Sea clone libraries. These findings differ from previously published phylogenies based on 16S rRNA gene analysis. Our data suggest that, in terms of their pigmentation, Synechococcus spp. represent three different lineages occupying different ecological niches in the underwater light spectrum. Strains from different lineages can coexist in light environments that overlap with their light absorption spectra.