Early IL-2 production by mouse dendritic cells is the result of microbial-induced priming
Early IL-2 production by mouse dendritic cells is the result of microbial-induced priming
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DOI：
10.4049/jimmunol.170.10.5075
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发表时间：
2003-05-15
影响因子：
4.4
通讯作者：
Ricciardi-Castagnoli, P
中科院分区：
文献类型：
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作者：
Granucci, F;Feau, S;Ricciardi-Castagnoli, P
树突状细胞（dc）是一种专业的apc，能够启动先天和适应性免疫反应来抵抗入侵的病原体。不同的特性，如高效的Ag加工机制，高水平的共刺激分子和肽- mhc复合物的表达，以及细胞因子的产生，有助于使dc成为幼稚T细胞反应的有效刺激物。最近，我们观察到dc能够在细菌刺激后产生IL-2，并且我们已经证明这种特殊的细胞因子是赋予早期细菌激活dc独特T细胞启动能力的关键分子。在目前的研究中，我们发现许多不同的微生物刺激，而不是炎症细胞因子，能够刺激DCs产生IL-2，这表明DCs可以区分细胞因子介导的炎症过程和实际存在的感染。在受到微生物刺激后产生IL-2的能力是体内多种DC亚型共有的特征，如CD8alpha（+）和CD8alpha（-）脾DC和表皮朗格汉斯细胞。当早期活化的dc与T细胞相互作用时，dc产生的IL-2在细胞-细胞接触部位富集，证实了dc来源的IL-2在T细胞活化中的重要性。
Dendritic cells (DCs) are professional APCs able to initiate innate and adaptive immune responses against invading pathogens. Different properties such as the efficient Ag processing machinery, the high levels of expression of costimulatory molecules and peptide-MHC complexes, and the production of cytokines contribute in making DCs potent stimulators of naive T cell responses. Recently we have observed that DCs are able to produce IL-2 following bacterial stimulation, and we have demonstrated that this particular cytokine is a key molecule conferring to early bacterial activated DCs unique T cell priming capacity. In the present study we show that many different microbial stimuli, but not inflammatory cytokines, are able to stimulate DCs to produce IL-2, indicating that DCs can distinguish a cytokine-mediated inflammatory process from the actual presence of an infection. The capacity to produce IL-2 following a microbial stimuli encounter is a feature shared by diverse DC subtypes in vivo, such as CD8alpha(+) and CD8alpha(-) splenic DCs and epidermal Langerhans cells. When early activated DCs interact with T cells, IL-2 produced by DCs is enriched at the site of cell-cell contact, confirming the importance of DCs-derived IL-2 in T cell activation.