Offspring of normal and diabetic rats fed saturated fat in pregnancy demonstrate vascular dysfunction
Offspring of normal and diabetic rats fed saturated fat in pregnancy demonstrate vascular dysfunction
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DOI：
10.1161/01.cir.98.25.2899
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发表时间：
1998-12-22
期刊：
影响因子：
37.8
通讯作者：
Poston, L
中科院分区：
文献类型：
--
作者：
Koukkou, E;Ghosh, P;Poston, L
背景-子宫内环境的紊乱可能为以后的生活中的疾病“编程”。在这项研究中，我们确定是否膳食脂肪补充剂和/或糖尿病在怀孕期间会对血管功能产生不利影响的后代。方法和结果-雌性Sprague-Dawley大鼠喂养繁殖饮食或饮食高饱和脂肪（30%wt/wt）交配前10天，整个怀孕期间，产后。在喂食30%脂肪饲料的15日龄断奶幼鼠的离体股动脉中，对乙酰胆碱的内皮依赖性舒张作用减弱。在对饲喂20%饱和脂肪的母鼠的60日龄后代进行的另一项研究中，也观察到内皮功能障碍和对去甲肾上腺素的收缩反应增强。在妊娠期间也给患有链脲佐菌素诱导的糖尿病的大鼠喂食饱和脂肪，与高脂肪喂养的正常母鼠的年轻后代相比，其15天龄后代的股动脉显示出内皮依赖性扩张受损，对去甲肾上腺素和血栓素模拟物U46619的收缩反应增强，在产后16天评估肠系膜动脉时，30%脂肪饮食也对母体糖尿病动物的血管功能有害，结论-妊娠期高脂饮食导致大鼠断奶和年轻成年后代的血管功能障碍。如果母鼠患有糖尿病，断奶后的幼鼠血管功能进一步恶化。
Background-Disturbances of the in utero environment may "program" for disease ill later life. In this study, we determined whether dietary fat supplementation and/or diabetes in pregnancy can adversely affect vascular function in the offspring.Methods and Results-Female Sprague-Dawley rats were fed a breeding diet or a diet high in saturated fat (30% wt/wt) for 10 days before mating, throughout pregnancy, and postpartum. Endothelium-dependent relaxation to acetylcholine was blunted in isolated femoral arteries of 15-day-old weanling pups from dams fed the 30%-fat diet. Endothelial dysfunction and enhanced constrictor responses to norepinephrine were also observed in an additional study of 60-day-old offspring of dams fed 20% saturated fat. Rats with streptozotocin-induced diabetes were also fed saturated fat during pregnancy, Femoral arteries from their 15-day-old offspring showed impairment of endothelium-dependent dilation and enhanced constrictor responses to norepinephrine and the thromboxane mimetic U46619 compared with young offspring of high-fat-fed normal dams, The 30%-fat diet was also deleterious to vascular function in the maternal diabetic animals when assessed in mesenteric arteries 16 days postpartum,Conclusions-A high-fat diet in pregnancy led to vascular dysfunction in rat weanlings and young adult offspring. Vascular function further deteriorated in weanlings if the maternal rat was diabetic.