Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment
Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment
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DOI：
10.1021/bm061050k
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发表时间：
2007-02-01
影响因子：
6.2
通讯作者：
Urban, Marek W.
中科院分区：
文献类型：
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作者：
Aumsuwan, Nattharika;Heinhorst, Sabine;Urban, Marek W.
对膨胀聚四氟乙烯（ePTFE）进行化学改性，以抑制金黄色葡萄球菌的生长。这是通过在马来酸酐（MA）存在下的微波等离子体反应来实现的，以在ePTFE表面上产生酸官能团，然后与200和600分子量的线性聚乙二醇（PEG）进行酯化反应。这种表面用于通过醚化反应与青霉素（PEN）进一步反应以产生抗微生物表面。这些反应导致表面形态变化，使用衰减全反射傅里叶变换红外光谱（ATR FT-IR）的光谱分析揭示了PEN和PEG官能度之间反应产生的酯键的形成。通过一系列实验评价抗菌活性，其中将PEN改性的ePTFE样品浸入液体金黄色葡萄球菌培养物中，并通过测量悬浮液在600 nm波长下的吸光度%来定量细菌生长。对于含有PEN-PEG-MA-ePTFE试样的溶液观察到最低吸光度，因此显示出对革兰氏阳性金黄色葡萄球菌的高效抗菌活性。据我们所知，这是第一项显示PEN-ePTFE表面改性对革兰氏阳性金黄色细菌有效的研究。
Expanded polytetrafluoroethylene (ePTFE) was chemically modified to retard the growth of Staphylococcus aureus bacteria. This was accomplished by microwave plasma reactions in the presence of maleic anhydride (MA) to create acid functional groups on ePTFE surfaces, followed by esterification reactions with 200 and 600 molecular weight linear polyethylene glycol (PEG). Such surfaces were utilized for further reactions with penicillin (PEN) through etherification reactions to create anti-microbial surfaces. These reactions resulted in surface morphological changes, and spectroscopic analysis using attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) revealed the formation of ester linkages resulting from reactions between PEN and PEG functionalities. Antibacterial activities were evaluated by a series of experiments where PEN-modified ePTFE specimens were immersed in a liquid aureus culture, and the bacteria growth was quantified by measuring % absorbance of the suspension at 600 nm wavelength. The lowest absorbance was observed for the solution containing PEN-PEG-MA-ePTFE specimens, thus showing highly effective anti-bacterial activity toward gram-positive Staphylococcus aureus bacteria. To our best knowledge, this is the first study that shows PEN-ePTFE surface modifications that are effective against gram-positive aureus bacteria.