Evidence for the involvement of Lfc and Tctex-1 in axon formation.
Evidence for the involvement of Lfc and Tctex-1 in axon formation.
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DOI：
10.1523/jneurosci.5420-09.2010
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发表时间：
2010-05-12
期刊：
影响因子：
--
通讯作者：
Cáceres A
中科院分区：
文献类型：
--
作者：
Conde C;Arias C;Robin M;Li A;Saito M;Chuang JZ;Nairn AC;Sung CH;Cáceres A
RhoA和Rac在神经元极化过程中起关键和相反的作用。我们现在表明，Lfc，鸟苷酸交换因子（GEF），定位于高尔基体和生长锥的发展中的神经元和负调控轴突发芽和轴突形成通过Rho信号通路。Tctex-1是一种通过调节肌动蛋白动力学和Rac活性参与轴突生长的动力蛋白轻链，与Lfc共定位并物理相互作用，从而抑制其GEF活性，降低Rho-GTP水平，并在神经突形成期间功能性拮抗Lfc。
RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.