Relationship of quantitative salivary levels of Streptococcus mutans and S-sobrinus in mothers to caries status and colonization of mutans streptococci in plaque in their 2.5-year-old children
Relationship of quantitative salivary levels of Streptococcus mutans and S-sobrinus in mothers to caries status and colonization of mutans streptococci in plaque in their 2.5-year-old children
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DOI：
10.1111/j.1600-0528.2009.00472.x
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发表时间：
2009-06-01
影响因子：
2.3
通讯作者：
Yonemitsu, M.
中科院分区：
文献类型：
--
作者：
Kishi, M.;Abe, A.;Yonemitsu, M.
本研究的目的是评估母亲唾液中变形链球菌和远缘链球菌的定量水平与其2.5岁儿童菌斑中变形链球菌(MS)的定植和龋病状况的关系。对54对母子进行口腔检查后，采集母亲唾液样本和儿童菌斑样本。用实时聚合酶链式反应(PCR)定量检测变形链球菌和远缘链球菌的含量(对数DNA拷贝数/毫升唾液)，同时用培养法检测菌斑样本中的MS。此外，54名儿童中有50名参与了为期两年的龋病患病率跟踪调查。在2.5岁的儿童中，MS(+)组的DFT阳性受试者的百分比和DFT的平均数量显著高于MS(-)组。为期两年的跟踪调查结果表明，MS(+)受试者的平均DFT数持续较高，为4.5年。根据母亲唾液中变形链球菌和远缘链球菌的数量水平，将2.5岁的儿童分为三组：母亲变形链球菌和远缘链球菌水平较低的儿童(第一组)；母亲变形链球菌水平较高(<4)和远缘链球菌水平较低的儿童(第二组)；以及母亲两者水平较高的儿童(<2组)。=2)(第3组)。在三组儿童中，MS(+)和DFT阳性儿童的百分比在第三组最高，在第一组最低。此外，多元Logistic回归分析表明，根据唾液中变形链球菌和远缘链球菌的水平对母亲进行分组是预测2.5岁儿童MS定植(OR=2.96)和患龋率(OR=9.39)的有效方法。用实时定量聚合酶链式反应检测母亲唾液中变形链球菌和远缘链球菌的水平与2.5岁时MS在牙菌斑中的定植和其子女的龋齿显著相关。因此，建议使用目前的临界值来确定母体中这两种生物的水平，作为一种有效的方法来指示多发性硬化症和儿童龋齿的母体传播风险。
The aim of this study was to assess the relationships of quantitative salivary levels of Streptococcus mutans and S. sobrinus in mothers with the colonization of mutans streptococci (MS) in plaque and caries status in their 2.5-year-old children. Furthermore, the dynamics of caries status in the children was evaluated in a 2-year follow-up survey.After oral examination of 54 mother-and-child pairs, the saliva samples from the mothers and the plaque samples from the children were collected. The levels (log DNA copies/ml saliva) of S. mutans and S. sobrinus were quantified using real-time polymerase chain reaction (PCR) assays, while MS in the plaque samples were detected using a cultivation method. In addition, 50 of the 54 children participated in a 2-year follow-up survey of caries prevalence.In the 2.5-year-old children, the percentage of dft-positive subjects and mean number of dft were significantly higher in the MS(+) group when compared with the MS(-) group. Findings from the 2-year follow-up survey indicated that MS(+) subjects had a persistently higher mean number of dft at 4.5 years. The 2.5-year-old children were divided into three groups based on the quantitative levels of salivary S. mutans and S. sobrinus in their mothers: those whose mothers had low levels of S. mutans (< 4 log DNA copies/ml) and S. sobrinus (< 2) (group 1); those whose mothers had a high level of S. mutans (>= 4) and low level of S. sobrinus (< 2) (group 2); and those whose mothers had high levels of both (>= 4 and >= 2, respectively) (group 3). Among the three groups, the percentages of MS(+) and dft-positive children were highest in group 3 and lowest in group 1. Furthermore, multiple logistic regression analyses revealed that grouping the mothers based on salivary level of S. mutans and S. sobrinus was an efficient means to predict both MS colonization (OR = 2.96) and prevalence of dental caries (OR = 9.39) in children at 2.5 years of age.In the 54 mother-and-child pairs tested, the maternal salivary levels of S. mutans and S. sobrinus determined by real-time PCR were significantly related to MS colonization in plaque as well as dental caries in their children at 2.5 years of age. Thus, determination of maternal levels of both organisms using the present cut-off values is proposed as an efficient method to indicate the risks of maternal transmission of MS and childhood dental caries.