Improved microchip design and application for in situ transmission electron microscopy of macromolecules.
Improved microchip design and application for in situ transmission electron microscopy of macromolecules.
复制标题
改进的微芯片设计和大分子原位透射电子显微镜的应用。
DOI：
10.1017/s1431927613013858
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Kelly,DeborahF
中科院分区：
文献类型：
--
作者：
Dukes,MadelineJ;Thomas,Rebecca;Damiano,John;Klein,KateL;Balasubramaniam,Sharavanan;Kayandan,Sanem;Riffle,JudyS;Davis,RicheyM;McDonald,SarahM;Kelly,DeborahF
了解大分子的基本性质促进了用于改善生物医学研究的新兴技术的发展。目前，迫切需要能够阐明生物医学试剂在天然环境中的功能的创新平台。为了满足这一需求，我们开发了一种原位方法来可视化纳米级生物医学相关大分子的动态行为。新设计的氮化硅设备包含集成的“微孔”被用来封闭在液体中的透射电子显微镜imaging purposes.We能够成功地检查新型磁共振成像造影剂，胶束悬浮液，脂质体载体车辆，转录病毒组件的活性大分子标本。对于每个测试的样本，集成的微孔充分地将大分子保持在离散的局部环境中，同时能够产生薄的液体层。
Understanding the fundamental properties of macromolecules has enhanced the development of emerging technologies used to improve biomedical research. Currently, there is a critical need for innovative platforms that can illuminate the function of biomedical reagents in a native environment. To address this need, we have developed an in situ approach to visualize the dynamic behavior of biomedically relevant macromolecules at the nanoscale. Newly designed silicon nitride devices containing integrated “microwells” were used to enclose active macromolecular specimens in liquid for transmission electron microscopy imaging purposes.We were able to successfully examine novel magnetic resonance imaging contrast reagents, micelle suspensions, liposome carrier vehicles, and transcribing viral assemblies. With each specimen tested, the integrated microwells adequately maintained macromolecules in discrete local environments while enabling thin liquid layers to be produced.