Structural genomics reveals EVE as a new ASCH/PUA-related domain.
Structural genomics reveals EVE as a new ASCH/PUA-related domain.
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DOI：
10.1002/prot.22287
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发表时间：
2009-05-15
影响因子：
2.9
通讯作者：
Rost, Burkhard
中科院分区：
文献类型：
--
作者：
Bertonati, Claudia;Punta, Marco;Fischer, Markus;Yachdav, Guy;Forouhar, Farhad;Zhou, Weihong;Kuzin, Alexander P.;Seetharaman, Jayaraman;Abashidze, Mariam;Ramelot, Theresa A.;Kennedy, Michael A.;Cort, John R.;Belachew, Adam;Hunt, John F.;Tong, Liang;Montelione, Gaetano T.;Rost, Burkhard
我们报告最近解决的结构基因组学财团，特别是东北结构基因组学财团（NESG）的几种蛋白质。在这项研究中考虑的蛋白质在它们的序列上有很大的不同，但它们共享一个相似的结构核心，其特征在于一个pseudobarrel五链β折叠。该核心对应于SCOP数据库中的类似于普阿域的体系结构。通过连接序列信息与结构知识，我们表征了这些蛋白质的一个新的亚组，我们建议与以前描述的普阿结构域，如普阿适当或ASCH明显不同。我们将这些新定义的域称为EVE。虽然EVE可能保留了普阿结构域结合RNA的能力，但现有的实验和计算数据表明，其分子功能和细胞功能的细节与其他普阿结构域样结构域不同。这项对EVE及其相关基因的研究说明了结构和基因组学的结合如何通过将映射到相同进化潜力的结构的聚宝盆连接起来来创造新的见解。单靠一级序列信息不足以揭示这些进化联系。
We report on several proteins recently solved by structural genomics consortia, in particular by the Northeast Structural Genomics consortium (NESG). The proteins considered in this study differ substantially in their sequences but they share a similar structural core, characterized by a pseudobarrel five-stranded beta sheet. This core corresponds to the PUA domain-like architecture in the SCOP database. By connecting sequence information with structural knowledge, we characterize a new subgroup of these proteins that we propose to be distinctly different from previously described PUA domain-like domains such as PUA proper or ASCH. We refer to these newly defined domains as EVE. Although EVE may have retained the ability of PUA domains to bind RNA, the available experimental and computational data suggests that both the details of its molecular function and its cellular function differ from those of other PUA domain-like domains. This study of EVE and its relatives illustrates how the combination of structure and genomics creates new insights by connecting a cornucopia of structures that map to the same evolutionary potential. Primary sequence information alone would have not been sufficient to reveal these evolutionary links.
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影响因子：
3.9
作者：
Aravind, L;Koonin, EV
通讯作者：
Koonin, EV
影响因子：
64.5
作者：
Ishitani, R;Nureki, O;Yokoyama, S
通讯作者：
Yokoyama, S
DOI：
10.1093/protein/7.11.1323
发表时间：
1994-11-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
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通讯作者：
GONNET, GH
影响因子：
5.8
作者：
Iyer, LM;Burroughs, AM;Aravind, L
通讯作者：
Aravind, L
影响因子：
4.8
作者：
Chaudhuri, BN;Sum, C;Yeates, TO
通讯作者：
Yeates, TO