Gold nanoparticle mediated detection of prostate cancer cells using photoacoustic flowmetry with optical reflectance.
Gold nanoparticle mediated detection of prostate cancer cells using photoacoustic flowmetry with optical reflectance.
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DOI：
10.1166/jbn.2010.1105
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发表时间：
2010-04
影响因子：
2.9
通讯作者：
J. Viator;Sagar K. Gupta;Benjamin S. Goldschmidt;K. Bhattacharyyal;R. Kannan;R. Shukla;P. Dale;E. Boote;K. Katti
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文献类型：
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作者：
J. Viator;Sagar K. Gupta;Benjamin S. Goldschmidt;K. Bhattacharyyal;R. Kannan;R. Shukla;P. Dale;E. Boote;K. Katti
血液中循环肿瘤细胞的存在与癌症患者的疾病状态相关。虽然我们已经成功地利用黑色素（黑色素瘤细胞中的天然光吸收剂）来诱导光声波用于肿瘤细胞检测，但非色素肿瘤细胞不具有足够的光学对比度来进行这种方法。例如，乳腺癌、前列腺癌和肺癌缺乏内在色素沉着，因此不会产生光声波。为了在非色素癌细胞中诱导光学对比度，我们将金纳米颗粒附着到前列腺癌细胞系上。这种光学吸收将使我们能够在光声流量计中检测此类细胞，该流量计旨在寻找血液样本中的循环肿瘤细胞。我们通过用金纳米颗粒标记前列腺癌细胞系 PC-3 来对其进行测试。我们确定了 470-570 nm 波长范围内的光声响应，以确定吸收峰。然后我们确定了悬浮在盐水中的连续稀释的 PC-3 细胞的响应。最后，我们展示了流量计中悬浮在白细胞中的 PC-3 细胞的光声响应，以证明我们检测流动下单个细胞的能力。
The presence of circulating tumor cells in the bloodstream has been correlated with disease state in cancer patients. While we have successfully exploited melanin, the natural light absorber in melanoma cells, to induce photoacoustic waves for tumor cell detection, non-pigmented tumor cells do not have sufficient optical contrast for such a method. For example, breast, prostate and lung cancers lack intrinsic pigmentation and thus do not generate photoacoustic waves. In order to induce optical contrast in non-pigmented cancer cells, we have attached gold nanoparticles to a prostate cancer cell line. This optical absorption will enable us to detect such cells in a photoacoustic flowmeter designed to find circulating tumor cells in blood samples. We tested a prostate cancer cell line, PC-3, by tagging them with gold nanoparticles. We determined the photoacoustic response over the wavelengths 470-570 nm to identify the absorption peak. We then determined the response from serial dilutions of PC-3 cells suspended in saline. Finally, we showed photoacoustic response from PC-3 cells suspended among white blood cells in the flow meter to demonstrate our ability to detect single cells under flow.