Expression profiling of integrins in lung cancer cells
Expression profiling of integrins in lung cancer cells
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DOI：
10.1016/j.prp.2009.07.005
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Liu, Tengfei
中科院分区：
文献类型：
--
作者：
Guo, Linlang;Zhang, Fan;Liu, Tengfei
整合素是由18个α和8个β亚基组成的异二聚体跨膜受体。α和β亚基的异源二聚体组成具有确定人肺癌肿瘤亚型的潜力。本研究的目的是探讨肺癌细胞中整合素的表达谱。应用cDNA微阵列、限制性内切酶基因表达分析（restriction analysis of gene expression，RFLP）和流式细胞术分析了一组肺癌细胞系A549（adenocarcinoma，ADC）、Calu-1（squamous carcinoma，SCC）、H1650（bronchioloalveolar carcinoma，BAC）和DMS-53（small cell lung cancer，SCLC）整合素的表达谱。79例肺癌标本用于进一步验证使用免疫组织化学的细胞系的数据。整合素明显地以细胞类型特异性方式表达，例如α 3在A549、Calu-1和H1650中表达，除了在DMS 53中，α 4在H1650中表达，α 5和β 1在所有细胞系中表达。用cDNA微阵列检测的整合素在蛋白质水平上均用ELISA和流式细胞术明确检测到。在所有肺癌标本中，α 3整合素在ADC、SCC和BAC中强表达，但在SCLC中不常见。α 4整合素仅在BAC中表达。α 5和β 1整合素在所有四种组织学类型的肺癌标本中表达。整合素α 3和α 4可作为腺癌、鳞状细胞癌和细支气管肺泡癌的诊断标志物。PCR是一种很有前途的研究基因表达谱的技术，如癌细胞中的整合素。(C)2009年Elsevier GmbH。All rights reserved.
Integrins are heterodimeric transmembrane receptors consisting of 18 alpha and 8 beta subunits. Heterodimer composition of alpha and beta subunits has a potential for determining tumor subtypes of human lung cancer. The purpose of this study was to investigate the expression profile of integrins in lung cancer cells. Expression profiling of integrins in a panel of lung cancer cell line, including A549 (adenocarcinoma, ADC), Calu-1 (squamous carcinoma, SCC), H1650 (bronchioloalveolar carcinoma, BAC) and DMS-53 (small cell lung cancer, SCLC), was analyzed by cDNA microarrays, restriction analysis of gene expression (RAGE) and flow cytometry. Seventy-nine lung cancer specimens were used to further validate the data from cell lines using immunohistochemistry. Integrins are obviously expressed in a cell type-specific manner, such as alpha 3 in A549, Calu-1 and H1650 except in DMS53, alpha 4 in H1650, alpha 5 and beta 1 in all cell lines. The integrins detected with cDNA microarrays were all unequivocally detected with RAGE and by flow cytometry at the protein level. In all lung cancer specimens, alpha 3 integrin was strongly expressed in ADC, SCC and BAC, but was infrequent in SCLC. alpha 4 integrin was solely expressed in BAC. alpha 5 and beta 1 integrins were expressed in all four histological types of lung cancer specimens. Integrin alpha 3 and alpha 4 may be useful as diagnostic markers for adenocarcinoma, squamous cell carcinoma and bronchioloalveolar carcinoma. RAGE is a promising technique for studying the expression profiles of genes, such as integrins in cancer cells. (C) 2009 Elsevier GmbH. All rights reserved.