Complex regulation of gene expression, photosynthesis and sugar levels by pathogen infection in tomato
Complex regulation of gene expression, photosynthesis and sugar levels by pathogen infection in tomato
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DOI：
10.1111/j.1399-3054.2004.00433.x
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发表时间：
2004-12-01
影响因子：
6.4
通讯作者：
Roitsch, T
中科院分区：
文献类型：
--
作者：
Berger, S;Papadopoulos, M;Roitsch, T
病原体对植物的感染导致防御反应的诱导以及碳水化合物代谢的变化。一方面，病原体试图操纵植物的碳水化合物代谢以获得其自身的优势。另一方面，植物必须重新组织碳通量，以确保对抗病原体。为了进一步研究病原菌侵染与植物碳水化合物代谢之间的关系，分析了活体营养型和坏死营养型两种病原菌对番茄基因表达、内源糖水平和光合作用的影响。光合基因表达下调假单胞菌和灰葡萄孢感染。与此相反，表达的库特异性基因编码的细胞壁转化酶和防御基因诱导的两种病原体。这些结果提供了证据的防御，汇和光合作用的基因表达在植物中响应这两种类型的病原体的共同调节。含油菜素内酯的植物修复剂ComCat增强了对B的抗性。并反向调节光合基因表达的抑制。B处理降低了内源糖水平，提高了己糖/蔗糖比。灰叶叶绿素荧光成像的应用揭示了病原体响应的时空异质性。在感染后24小时，光合电子传递的抑制被限制在感染部位的直接附近，这是由一圈增加的光合活性包围。剩余叶片的光合作用在此阶段不受影响。这些结果表明，叶绿素荧光成像的有用性的复杂的时空变化的评估和相关的其他类型的确定，例如基因表达的定义的领域。
The infection of plants with pathogens results in the induction of defence reactions as well as changes in carbohydrate metabolism. On the one hand, the pathogen attempts to manipulate the carbohydrate metabolism of the plant for its own advantage. On the other, the plant has to reorganize carbon fluxes to ensure fight against the pathogen. In order to further investigate the connection between pathogen infection and carbohydrate metabolism, the effects of two types of pathogen, biotrophic and necrotrophic, on gene expression, endogenous sugar levels and photosynthesis of tomato plants were analysed. Photosynthetic gene expression was downregulated on infection with Pseudomonas syringae and Botrytis cinerea. In contrast, expression of a sink-specific gene encoding a cell wall invertase and of defence genes was induced by both pathogens. These results provide evidence for a co-regulation of defence, sink and photosynthetic gene expression in planta in response to both types of pathogen. The brassinosteroid-containing plant restorative ComCat enhanced resistance against B. cinerea and counter-regulated the repression of photosynthetic gene expression. Endogenous sugar levels decreased and the hexose to sucrose ratio increased on treatment with B. cinerea. The application of chlorophyll fluorescence imaging revealed the spatio-temporal heterogeneity of the pathogen response. At 24 h after infection, inhibition of photosynthetic electron transport was restricted to the direct vicinity of the infection site, which was surrounded by a circle of increased photosynthetic activity. The photosynthesis of the remaining leaf was not affected at this stage. These results show the usefulness of chlorophyll fluorescence imaging for the assessment of the complex spatio-temporal changes and for the definition of the areas relevant for other types of determination, e.g. gene expression.