His-tag impact on structure
His-tag impact on structure
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DOI：
10.1107/s0907444906052024
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发表时间：
2007-03-01
期刊：
影响因子：
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通讯作者：
DeLucas, Lawrence J.
中科院分区：
文献类型：
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作者：
Carson, Mike;Johnson, David H.;DeLucas, Lawrence J.
晶体学家越来越多地测定包含组氨酸标签的蛋白质构建体的结构。许多人想当然地认为这些标签对天然结构几乎没有影响。本文对有组氨酸标签和无组氨酸标签的晶体结构进行了调查和比较。据观察，在不到10%的带标签序列中，实际精修的符合电子密度的标签残基才会出现。然而，当这种情况发生时，可以观察到更高分辨率的晶体。结果表明，这些纯化标签通常对天然蛋白质的结构没有显著影响。分辨率和R因子不受影响，但总体B因子略高。建议在PDB格式中添加额外注释以使标签定义明确。
Crystallographers are increasingly determining structures of protein constructs that include His tags. Many have taken for granted that these tags have little effect on the native structure. This paper surveys and compares crystal structures with and without His tags. It is observed that actual refined tag residues fitted into density occur in less that 10% of the tagged sequences. However, higher resolution crystals are observed when this occurs. It is shown that these purification tags generally have no significant effect on the structure of the native protein. Resolution and R factors are not affected, but the overall B factors are slightly higher. Additional annotation in the PDB format to make tag definition explicit is suggested.