DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
复制标题
DOI：
10.1073/pnas.88.21.9755
复制
发表时间：
1991-11
影响因子：
11.1
通讯作者：
M. White;M. Wierdl;P. Detloff;T. D. Petes
中科院分区：
文献类型：
--
作者：
M. White;M. Wierdl;P. Detloff;T. D. Petes
与其他真核生物一样，在酿酒酵母中，基因组的某些区域比其他区域具有更高的减数分裂重组率。我们在下文中表明，RAP 1蛋白与HIS 4基因上游位点的结合对于该位点的高减数分裂（但不是有丝分裂）重组率是必需的。在HIS 4的RAP 1结合位点的突变导致重组减少; RAP 1的过度产生导致HIS 4的重组增加，高于野生型水平。
In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.