Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells.
Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells.
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DOI：
10.1097/shk.0000000000000066
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发表时间：
2013-12
期刊：
影响因子：
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通讯作者：
Wischmeyer PE
中科院分区：
文献类型：
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作者：
Khailova L;Baird CH;Rush AA;McNamee EN;Wischmeyer PE
最近的临床试验表明，在危重疾病中使用鼠李糖乳杆菌 GG (LGG) 有可能减少医院感染并改善临床结果。然而，LGG 介导的疾病和损伤后益处的机制仍然难以捉摸。本研究的目的是确定 LGG 治疗对铜绿假单胞菌引起的肺炎小鼠模型的生存和肺损伤的影响。由于增加 T 调节 (Treg) 细胞数量已被证明可以改善实验性肺炎的治疗结果，我们研究了 Treg 细胞在益生菌介导的益处中的潜在作用。对 FVB/N 小鼠进行气管内注射铜绿假单胞菌或盐水，并在手术前立即接受 LGG 或载体。通过聚合酶链反应、蛋白质印迹和流式细胞术评估肺部 T 调节细胞反应。与用盐水治疗的对照肺炎小鼠相比（55% LGG 对比 14% 对照），用 LGG 治疗的小鼠的 7 天生存率显着提高（P < 0.01）。益生菌组的生存优势与支气管肺泡灌洗液中细菌计数的减少、全身炎症反应标志物的减少以及肺部病理学的改善有关。 Treg 细胞标记 Foxp3 水平升高证明，益生菌治疗影响了肺炎小鼠肺部的免疫反应。这些数据表明，早期给予 LGG 可改善铜绿假单胞菌引起的肺炎的预后。 LGG 对 Treg 细胞的作用可能在这种保护中发挥作用。
Recent clinical trials show Lactobacillus rhamnosus GG (LGG) administration in critical illness has the potential to reduce nosocomial infections and improve clinical outcome. However, the mechanism(s) of LGG-mediated benefit following illness and injury remain elusive. The aim of this study was to determine the effect of LGG treatment on survival and lung injury in a mouse model of Pseudomonas aeruginosa–induced pneumonia. As increased T regulatory (Treg) cell numbers have been shown to improve outcome in experimental pneumonia, we examined the potential role of Treg cells in probiotic-mediated benefit. FVB/N mice were subjected to intratracheal injection of either P. aeruginosa or saline and received LGG or vehicle immediately before procedure. T regulatory cell responses in the lung were evaluated by polymerase chain reaction, Western blotting, and flow cytometry. Mice treated with LGG had significantly improved 7-day survival (P < 0.01) compared with saline-treated control pneumonia mice (55% LGG vs. 14% control). The survival advantage was associated with reduced bacterial counts in bronchoalveolar lavage and with decreased markers of the systemic inflammatory response and improved lung pathology in the probiotic group. Probiotic treatment influenced immune response in the lungs of mice with pneumonia as demonstrated by increased levels of Treg cell marker Foxp3. These data demonstrate that early administration of LGG improves outcome following P. aeruginosa–induced pneumonia. An effect of LGG on Treg cells may play a role in this protection.