Circular RNA CircHIPK3 Elevates CCND2 Expression and Promotes Cell Proliferation and Invasion Through miR-124 in Glioma
Circular RNA CircHIPK3 Elevates CCND2 Expression and Promotes Cell Proliferation and Invasion Through miR-124 in Glioma
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DOI：
10.3389/fgene.2020.01013
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发表时间：
2020-08-28
影响因子：
3.7
通讯作者：
Liu, Xianzhi
中科院分区：
文献类型：
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作者：
Liu, Zengjin;Guo, Shewei;Liu, Xianzhi
胶质瘤是一种中枢神经系统恶性肿瘤，发病率高，预后差。环状RNA HIPK3 （circHIPK3）是一种与癌症进展相关的环状RNA （circRNA）。然而，circHIPK3在胶质瘤中的作用尚不清楚。本研究的目的是探讨circHIPK3在胶质瘤中的作用及其机制。采用qRT-PCR方法检测circHIPK3在胶质瘤细胞系中的表达模式。CCK-8法检测细胞增殖。采用Transwell实验评估细胞迁移和侵袭。我们的研究结果表明，circHIPK3在胶质瘤组织和细胞系中的表达显著上调。在体外实验中，下调circHIPK3可显著抑制胶质瘤细胞的增殖、迁移和侵袭。此外，我们证明circHIPK3像海绵一样吸收miR-124并促进CCND2的表达。综上所述，我们的研究结果表明circHIPK3通过海绵化miR-124调节胶质瘤中CCND2的表达而具有致癌作用。这些发现为揭示circHIPK3在胶质瘤发展中的作用提供了有利的证据。
As a malignant tumor of the central nervous system, glioma exhibits high incidence and poor prognosis. Circular RNA HIPK3 (circHIPK3) is a circular RNA (circRNA) related to cancer progression. However, the role of circHIPK3 in gliomas remains unclear. The purpose of this study was to investigate the role of circHIPK3 in gliomas and its mechanism. The qRT-PCR method was used to determine the expression pattern of circHIPK3 in glioma cell lines. CCK-8 assay was used to detect cell proliferation. Cell migration and invasion were evaluated using the Transwell assay. Our results showed that circHIPK3 expression was significantly up-regulated in glioma tissues and cell lines.In vitro, the down-regulation of circHIPK3 significantly inhibited the proliferation, migration and invasion of glioma cells. Besides, we demonstrated that circHIPK3 acted as a sponge to absorb miR-124 and promoted CCND2 expression. In summary, our results indicated that circHIPK3 had carcinogenic effects by regulating the expression of CCND2 in glioma by sponging miR-124. These findings provided favorable evidence to reveal the role of circHIPK3 in the development of gliomas.