INTERACTION OF CYTOCHROME-C WITH CARDIOLIPIN - AN INFRARED SPECTROSCOPIC STUDY
INTERACTION OF CYTOCHROME-C WITH CARDIOLIPIN - AN INFRARED SPECTROSCOPIC STUDY
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DOI：
10.1016/0301-4622(94)00151-9
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发表时间：
1995-05-01
影响因子：
3.8
通讯作者：
SWANSON, JM
中科院分区：
文献类型：
--
作者：
CHOI, SH;SWANSON, JM
用红外光谱法比较了细胞色素c （cyt c）与线粒体膜上的主要阴离子磷脂——心磷脂和二油基磷脂酰甘油（DOPG）的相互作用。脂质羰基的傅里叶自反卷积红外光谱表明，cyt c（3+）和cyt c（2+）扰乱和/或使心磷脂双层的界面区域脱水。只有一个轻微的扰动，如果有的话，观察到在界面区域的DOPG双层。然而，DOPG的磷酸盐头部区域受到cyt c（3+）的干扰，这在心磷脂中没有检测到。结果表明，两种氧化还原状态下的细胞色素c都能部分渗透到心磷脂中，但不能渗透到DOPG双分子层中。cyt c与心磷脂和DOPG的相互作用主要为疏水性和静电性。酰胺I区的傅里叶自反卷积红外光谱显示，约10%的cyt c(3+) ct-螺旋在与心磷脂双层结合时展开成随机线圈。然而，当cyt c（3+）与DOPG双分子层结合时，只检测到非常轻微的二次结构变化。
The interactions of cytochrome c (cyt c) with cardiolipin, a major anionic phospholipid of mitochondrial membranes, and dioleoylphosphatidylglycerol (DOPG), have been compared by infrared (IR) spectroscopy. The Fourier self-deconvoluted IR spectra of the lipid carbonyl groups indicate that both cyt c(3+) and cyt c(2+) perturb and/or dehydrate the interfacial region of cardiolipin bilayers. Only a slight perturbation, if any, is observed in the interfacial region of DOPG bilayers. However, the phosphate head region of DOPG is perturbed by cyt c(3+), which was not detected in cardiolipin. The results suggest that cytochrome c in both redox states can partially penetrate into cardiolipin but not into DOPG bilayers. The interaction of cyt c with cardiolipin and DOPG is mainly hydrophobic and electrostatic, respectively. The Fourier self-deconvoluted IR spectra in the amide I region reveal that ca. 10% of the cyt c(3+) ct-helix unfolds to random coil upon binding to cardiolipin bilayers. However, only very slight secondary structural changes, if any, were detected when cyt c(3+) binds to DOPG bilayers.