Calcium dependency of the inhibitory effect of antidiuretic hormone on in vitro renin secretion in rats.
Calcium dependency of the inhibitory effect of antidiuretic hormone on in vitro renin secretion in rats.
复制标题
抗利尿激素对大鼠体外肾素分泌抑制作用的钙依赖性。
DOI：
10.1113/jphysiol.1981.sp013729
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Churchill,PC
中科院分区：
文献类型：
--
作者：
Churchill,PC
1.合成精氨酸加压素（ADH或抗利尿激素）抑制大鼠肾皮质切片的肾素分泌速率。反应呈浓度依赖性，在0.1 U时最大。毫升。-1。2.钙耗竭（孵育片在培养基中含有Na 2 EGTA和无添加氯化钙）刺激肾素分泌，并最终取消ADH的抑制作用。这两种影响都是可逆的。3.钙拮抗剂D-600（0.5 μ M）可逆转K去极化对分泌速率的抑制作用，但对去极化切片上的分泌速率无影响。在存在0.5 microM-D-600的情况下，ADH抑制去极化或非去极化切片的分泌速率。4.这些结果证实和扩展以前的观察表明，钙发挥抑制性偶联作用的控制肾素分泌。此外，他们还表明，虽然通过电压敏感性Ca通道的Ca内流影响肾小球细胞的分泌活性，但ADH激活了Ca动员的独立途径。
1. Synthetic arginine‐vasopressin (ADH or antidiuretic hormone) inhibited renin secretory rate of rat renal cortical slices. The response was concentration‐dependent and was maximal at 0.1 U . ml.‐1. 2. Ca depletion (incubation of slices in medium containing Na2EGTA and no added CaCl2) stimulated renin secretion and eventually abolished the inhibitory effect of ADH. Both these effects were reversible. 3. The Ca antagonist D‐600, at 0.5 microM, reversed the inhibitory effect of K depolarization on secretory rate but had no effect on secretory rate on on‐depolarized slices. In the presence of 0.5 microM‐D‐600, ADH inhibited the secretory rate of either depolarized or non‐depolarized slices. 4. These results confirm and extend previous observations suggesting that Ca plays an inhibitory coupling role in the control of renin secretion. Moreover they suggest that although Ca influx through voltage‐sensitive Ca channels influences the secretory activity of juxtaglomerular cells, ADH activates an independent pathway for Ca mobilization.