Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR
Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR
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DOI：
10.1039/d2cc05576g
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发表时间：
2022-11-29
影响因子：
4.9
通讯作者：
Katahira, Masato
中科院分区：
文献类型：
--
作者：
Eladl, Omar;Yamaoki, Yudai;Katahira, Masato
我们引入了一种由E. coli转录到HeLa细胞中。我们成功地记录了第一个异源二维细胞内NMR光谱，这使得研究RNA适体与烟酰胺在活体人类细胞中的相互作用成为可能。
We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution.