Saccharomyces cerevisiae lipid droplet associated enzyme Ypr147cp shows both TAG lipase and ester hydrolase activities
Saccharomyces cerevisiae lipid droplet associated enzyme Ypr147cp shows both TAG lipase and ester hydrolase activities
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DOI：
10.2323/jgam.2017.08.001
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发表时间：
2018-01-01
影响因子：
1.2
通讯作者：
Bondili, J. S.
中科院分区：
文献类型：
--
作者：
Kumar, Naresh M.;Thunuguntla, V. B. S. C.;Bondili, J. S.
酿酒酵母Ypr 147 cp被发现定位于脂滴，但Ypr 147 cp的生理作用仍然未知。Ypr 147 cp的序列分析揭示了α/β水解酶结构域沿着保守的GXSXG脂肪酶基序。重组Ypr 147 cp同时具有甘油三酯脂肪酶和酯水解酶的活性。当与野生型（WT）相比时，YPR 147 C的敲除导致TAG在ypr 147 c Delta中的积累。此外，ypr 147 δ细胞的透射电子显微镜分析揭示了更多数量的脂质体，证明了TAG含量的增加，并且在ypr 147 c δ中过表达YPR 147 C后挽救了表型。此外，脂质分析证实了来源于中性和磷脂的脂肪酸在ypr 147 c Delta细胞中的蓄积。基于这些结果，Ypr 147 cp被鉴定为具有酯水解能力的脂滴相关三酰甘油脂肪酶沿着。
Saccharomyces cerevisiae Ypr147cp was found localized to lipid droplets but the physiological role of Ypr147cp remains unknown. Sequence analysis of Ypr147cp revealed an alpha/beta hydrolase domain along with the conserved GXSXG lipase motif. Recombinant Ypr147cp showed both triacylglycerol lipase and ester hydrolase activities. Knock out of YPRJ47C led to accumulation of TAG in ypr147c Delta when compared to wild type (WT). In addition, transmission electron microscopic analysis of ypr147 Delta cells revealed a greater number of lipid bodies, justifying the increase in TAG content, and the phenotype was rescued upon overexpression of YPR147C in ypr147c Delta. Moreover, the lipid profiling confirmed the accumulation of fatty acids derived from neutral and phospholipids in ypr147c Delta cells. Based on these results, Ypr147cp is identified as a lipid droplet associated triacylglycerol lipase along with an ester hydrolyzing capacity.