Exosomal circRNAs as novel potential biomarkers for colorectal adenoma.
Exosomal circRNAs as novel potential biomarkers for colorectal adenoma.
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DOI：
10.4149/neo_2021_210718n986
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发表时间：
2022-01
期刊：
影响因子：
3
通讯作者：
Shaobing Cheng;Sen Lu;Fanlong Liu
中科院分区：
文献类型：
--
作者：
Shaobing Cheng;Sen Lu;Fanlong Liu
越来越多的证据表明，外泌体 miRNA、lncRNA 和 circRNA 在肿瘤生物学中发挥着多效性作用。外泌体介导的细胞间通讯被认为是结直肠癌恶性进展的关键因素。然而，外泌体衍生的circRNA在结直肠腺瘤的生物学功能和临床意义中的重要性仍然难以捉摸。在这项研究中，我们的目的是利用高通量测序鉴定从结直肠腺瘤患者血浆中分离的外泌体中改变的 circRNA 表达谱。通过蛋白质印迹、透射电子显微镜和纳米视觉测定证实了外泌体。测序数据显示，与对照组相比，结直肠腺瘤患者中有413个差异表达的circRNA，其中112个上调，301个下调。进行 GO 分析和 circRNA-miRNA-mRNA 网络来预测 circRNA 的潜在功能，并证明结直肠腺瘤中的假定机制。总的来说，我们的研究结果表明，血浆外泌体 circRNA 可能是检测结直肠腺瘤的潜在非侵入性生物标志物，并为结直肠腺瘤-癌序列提供了新的见解。
Growing evidences have revealed that exosomal miRNAs, lncRNAs, and circRNAs play a pleiotropic role in tumor biology. Cell-cell communication mediated by exosomes has been considered to be a key factor in the malignant progression of colorectal cancer. However, the importance of exosome-derived circRNAs in the biological function and clinical significance of colorectal adenoma remains elusive. In this study, we aimed to identify altered circRNA expression profiles in exosomes isolated from plasma of patients with colorectal adenoma using high-throughput sequencing. Exosomes were confirmed by western blotting, transmission electron microscopy, and nanosight assay. The sequencing data indicated that there are 413 differentially expressed circRNAs including 112 upregulated and 301 downregulated circRNAs in colorectal adenoma patients compared with controls. GO analysis and the circRNA-miRNA-mRNA network were performed to predict the potential function of circRNAs, and demonstrate the putative mechanisms in colorectal adenoma. Collectively, our findings revealed that plasma exosomal circRNAs may be a potential noninvasive biomarker for the detection of colorectal adenoma, and provided new insights into colorectal adenoma-carcinoma sequence.